THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES
THE MITOGENIC EFFECT OF LIPOPOLYSACCHARIDE ON BONE MARROW-DERIVED MOUSE LYMPHOCYTES
复制标题
脂多糖对小鼠骨髓源性淋巴细胞的促有丝分裂作用
DOI：
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发表时间：
1973
影响因子：
15.3
通讯作者：
O. Lüderitz
中科院分区：
文献类型：
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作者：
J. Andersson;F. Melchers;C. Galanos;O. Lüderitz
从大肠杆菌和沙门氏菌的不同突变株中分离的具有不同结构的脂多糖以及这些脂多糖的化学降解产物已被用于研究脂多糖分子的哪一部分对骨髓来源的小鼠淋巴细胞产生促有丝分裂作用。在由脂质A、核心多糖和O-多糖抗原组成的脂多糖结构内，发现脂质A是促有丝分裂部分。脂质A的促有丝分裂作用，由磷酸化葡萄糖胺二糖单位与酯和酰胺连接的脂肪酸，碱处理后，这将消除酯连接的脂肪酸丢失。插入的脂质A部分的脂多糖到骨髓来源的淋巴细胞的质膜的脂质双层进行了讨论，作为初始的促有丝分裂作用。
Lipopolysaccharides with different structure, isolated from different mutant strains of Escherichia coli and Salmonella bacteria, and chemical degradation products of these lipopolysaccharides have been employed to investigate which part of the lipopolysaccharide molecule exerts mitogenic effects on bone marrow-derived mouse lymphocytes. Within the structure of lipopolysaccharide consisting of lipid A, a core polysaccharide, and the O-polysaccharide antigen, lipid A was found to be the mitogenic part. The mitogenic effect of lipid A, consisting of phosphorylated glucosamine disaccharide units with ester- and amide-linked fatty acids, was lost after alkali treatment, which removes ester-linked fatty acids. Insertion of the lipid A portion of lipopolysaccharides into the lipid bilayer of the plasma membranes of bone marrow-derived lymphocytes is discussed as the initial mitogenic action.