Expression of Transient Receptor Potential Melastatin 7 Up-regulated in the Early Stage of Renal Ischemia-Reperfusion
Expression of Transient Receptor Potential Melastatin 7 Up-regulated in the Early Stage of Renal Ischemia-Reperfusion
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DOI：
10.1016/j.transproceed.2011.11.068
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发表时间：
2012-06-01
影响因子：
0.9
通讯作者：
Xiang, F.
中科院分区：
文献类型：
--
作者：
Meng, Z.;Wang, X.;Xiang, F.
背景。瞬时受体电位美拉抑素7 （TRPM7）是一种具有激酶和离子通道活性的双功能蛋白，于2001年首次报道。后来的研究表明，TRPM7的功能与癌症、缺血性损伤和高血压有关。我们检测了TRPM7在肾缺血再灌注损伤（RIRI）中的表达是否改变。选取体重220 ~ 250 g的雄性sd大鼠70只，随机分为7组，每组7只；每组设3只假手术组。取右肾后，大鼠左肾动脉闭塞45分钟，再灌注1、12、24、48、72、96小时。然后用实时聚合酶链反应（RT-PCR）和Western blot检测trpm7信使RNA （mRNA）的表达。与对照组相比，缺血再灌注后24小时trpm7 mRNA的表达明显上调。我们以对照组trpm7 mRNA表达量为基线（1.0），1小时、12小时、24小时、48小时、72小时、96小时分别为2.83、3.16、3.95、0.31、0.82、1.13 （n = 7, P < 0.05）。TRPM7蛋白表达与mRNA表达趋势一致。TRPM7蛋白的改变主要发生在小管细胞中。肾缺血后24小时，trpm7 mRNA表达和蛋白水平上调。
Background. Transient receptor potential melastatin 7 (TRPM7), a bifunctional protein with kinase and ion channel activities, was first reported in 2001. Later studies suggested that the functions of TRPM7 related to cancer, ischemic injury, and hypertension.Methods. We examined whether TRPM7 expression changed in renal ischemia-reperfusion injury (RIRI). Seventy male Sprague-Dawley rats weighing 220-250 g were randomly divided into 7 groups of 7 rats each; every group had a sham-operated cohort of 3 rats. After the right kidney was removed, the rats underwent 45 minutes of left renal artery occlusion followed by reperfusion for 1, 12, 24, 48, 72, or 96 hours. There after the expression of trpm7 messenger RNA (mRNA) was measured using real-time polymerase chain reaction (RT-PCR) and the protein using Western blot.Results. Compared with the control group, the expression of trpm7 mRNA was significantly up-regulated at 24 hours after ischemia-reperfusion. We set the expression of trpm7 mRNA of the control group as the baseline (1.0), with the 1-hour, 12-hour, 24-hour, 48-hour, 72-hour and 96-hours showing 2.83, 3.16, 3.95, 0.31, 0.82, and 1.13, respectively (n = 7; P < .05). TRPM7 protein expression showed the same trends as the mRNA. The main change in TRPM7 protein was located in tubular cells.Conclusions. The expressions of trpm7 mRNA and protein levels was up-regulated at 24 hours after renal ischemia.