S-related protein can be recombined with self-compatibility in interspecific derivatives ofLycopersicon
S-related protein can be recombined with self-compatibility in interspecific derivatives ofLycopersicon
复制标题
番茄种间衍生物中S相关蛋白可自相容性重组
DOI：
10.1007/bf00553620
复制
发表时间：
1995
影响因子：
2.4
通讯作者：
B. Rivers
中科院分区：
文献类型：
--
作者：
R. Bernatzky;Richard H. Glaven;B. Rivers
已鉴定出参与番茄自交不亲和反应的花柱蛋白，并将其定位于控制自交不亲和反应的位点（S位点）。栽培番茄的自交亲和（SC）物种esculentum不显示这些蛋白质。SCL之间的混合。esculentum和SIL.尽管这些个体携带含有L.蔬菜在回交后代中，杂种与L.在Esculentum中，来自mL的S等位基因的存在之间存在强相关性。多毛症和自身不育症。然而，这种关系在许多回交（BC）后代中是不偶联的。在两个自交能育的BC个体中，SI反应似乎不存在，这两个个体是L的S等位基因杂合的。hirsutum，基于孟德尔分离的紧密连锁的DNA标记，CD15在自交后代。在这些后代中，自交可育的个体是L.该连锁标记的hirsutum等位基因也被确定为与L的S相关蛋白纯合。通过与L.蔬菜因此，产生了功能性S等位基因纯合但自交能育的植物。这一结果和其他证据表明，S相关蛋白不足以引起L的自交不亲和反应。而这其中，有一个突变点，就是L。esculentum其他地方比theSlocus导致自我兼容性。
Stylar proteins involved in the self-incompatible (SI) response ofLycopersicon hirsutumhave been identified and mapped to the locus that controls SI (S locus).L. esculentum, a self-compatible (SC) species of cultivated tomato, does not display these proteins. Hybrids between SCL. esculentumand SIL. hirsutumare self-sterile despite these individuals bearing pollen containing theSallele ofL. esculentum. In progeny derived from backcrossing the hybrids toL. esculentum, there was a strong correlation between the presence of theSallele fromL. hirsutumand self-infertility. However, this relationship was uncoupled in a number of backcross (BC) progeny. The SI response appeared to be nonexistent in two self-fertile BC individuals that were heterozygous for theSallele ofL. hirsutum, based on Mendelian segregation of a tightly linked DNA marker,CD15, in selfed progeny. Among these progeny self-fertile individuals that were homozygous for theL. hirsutumallele of the linked marker were also determined to be homozygous for anS-related protein ofL. hirsutumthrough test crosses withL. esculentum. Therefore, plants were produced that were homozygous for a functionalSallele but were self-fertile. This result and other evidence suggest that theS-related proteins are not sufficient to elicit a self-incompatible response inL. esculentumand that there is a mutation(s) inL. esculentumsomewhere other than theSlocus that leads to self-compatibility.