Amelioration effect of bovine casein glycomacropeptide on ulcerative colitis in mice
Amelioration effect of bovine casein glycomacropeptide on ulcerative colitis in mice
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DOI：
10.1080/09540105.2015.1018874
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发表时间：
2015-03
影响因子：
3
通讯作者：
Zhu Ming;Yuchen Jia;Yali Yan;Guangchang Pang;Qingsen Chen
中科院分区：
文献类型：
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作者：
Zhu Ming;Yuchen Jia;Yali Yan;Guangchang Pang;Qingsen Chen
探讨酪蛋白糖巨肽对恶唑酮诱导的小鼠溃疡性结肠炎的改善作用机制。将BALB/c小鼠分为4组：（1）健康对照组，（2）溃疡性结肠炎模型对照组，（3）酪蛋白糖巨肽（CGMP）溃疡性结肠炎模型组，（4）柳氮磺胺吡啶（SASP）溃疡性结肠炎模型组。给药结束时，采用流式细胞仪微球阵列和酶联免疫吸附法检测血清IL-1β、IL-2、IL-4、IL-5、IFN-γ、TNF-α和IL-10水平，采用Western blotting法检测丝裂原活化蛋白激酶p38和NF-κB p65水平。结果表明，NF-κB和MAPK信号通路失活，血清IL-1β、IL-5、IFN-γ和TNF-α水平下调，IL-10产生上调。这些变化可能与牛酪蛋白糖巨肽改善恶唑酮诱导的溃疡性结肠炎有关。
To explore the mechanism for amelioration effect of bovine casein glycomacropeptide on oxazolone-induced ulcerative colitis in mice. BALB/c mice were divided into four groups as follows: (1) healthy control, (2) ulcerative colitis model control, (3) casein glycomacropeptide-supplemented ulcerative colitis model (CGMP group), and (4) sulfasalazine-supplemented ulcerative colitis model (SASP group). At the end of the administration period, serum IL-1β, IL-2, IL-4, IL-5, IFN-γ, TNF-α, and IL-10 were measured by cytometric bead array and enzyme-linked immunosorbent assay, and mitogen-activated protein kinase p38 and NF-κB p65 were determined by Western blotting. The results demonstrated the inactivation of NF-κB and MAPK signaling pathways and the downregulation of the serum levels of IL-1β, IL-5, IFN-γ, and TNF-α and upregulation of IL-10 production. These changes may be associated with amelioration of oxazolone-induced ulcerative colitis by bovine casein glycomacropeptide.