Mitochondrial disturbances in HIV pregnancies-猫眼课题宝

DOI：

10.1097/qad.0000000000000486

发表时间：

2015-01-02

期刊：

AIDS

影响因子：

3.8

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

Moren, Constanza;Noguera-Julian, Antoni;Fortuny, Claudia

背景资料：线粒体的后果胎儿暴露于艾滋病毒感染和抗逆转录病毒药物可以进一步investigated.Objective：本研究的主要目的是评估maternofetal线粒体紊乱在艾滋病毒感染和抗逆转录病毒药物管理在人类妊娠的病因基础次优围产期临床feature.Design：横断面，前瞻性，观察性，探索性和对照研究。收集了35名艾滋病毒感染孕妇和17名对照的临床/流行病学数据。采用真实的时间-聚合酶链反应（realtime-PCR）法测定白细胞线粒体DNA（mtDNA）和RNA（mtDNA）含量，分光光度法测定酶活性和含量。遗传功能，maternofetal和分子临床relationships进行了assessed.Results：出生体重较低的婴儿从HIV感染的母亲与对照组相比。在HIV病例中，线粒体DNA值略有下降，但未达到统计学显著性。HIV感染母亲的MTRNA值较低。类似地，二元复合物II+III酶活性在HIV感染的母亲（44.45 +/- 3.77%）和她们的婴儿（48.79 +/- 3.41%）中均降低至50%（P = 0.001和P < 0.001）。HIV感染母亲和婴儿的总体CI+III+IV酶活性较低（90.43 ± 2.39%和51.16 ± 9.30%）（P < 0.005和P < 0.05）。MtDNA含量与母亲和婴儿的功能相关。maternofetal参数相关的遗传和功能levels.Conclusion：HAART毒性造成线粒体损伤的HIV感染的孕妇和新生儿，目前在遗传和功能水平与maternofetal相关。(C)2014威科健康垂直酒吧Lippincott威廉姆斯& Wilkins

Background: Mitochondrial consequences from foetal exposure to HIV infection and antiretrovirals could be further investigated.Objective: The main objective of this study was to evaluate maternofoetal mitochondrial disturbances in HIV infection and antiretroviral administration in human pregnancies as the aetiopathogenic basis of suboptimal perinatal-clinical features.Design: Cross-sectional, prospective, observational, exploratory and controlled study.Methods: Clinical/epidemiological data of 35 HIV-infected pregnant women and 17 controls were collected. Mitochondrial DNA (mtDNA) and RNA (mtRNA) content (real time-PCR), enzymatic activities and content (spectrophotometry) were measured in leucocytes. Genetic-functional, maternofoetal and molecular-clinical correlations were assessed.Results: Birth weight was lower in infants from HIV-infected mothers compared with controls. MtDNA values were slightly decreased in HIV cases, although not reaching statistical significance. MtRNA values were lower in HIV-infected mothers. Similarly, binary complex II+III enzymatic activity decreased to 50% in both HIV-infected mothers (44.45 +/- 3.77%) and their infants (48.79 +/- 3.41%) (P = 0.001 and P < 0.001). Global CI+III+IV enzymatic activity was lower in HIV-infected mothers and infants (90.43 +/- 2.39% and 51.16 +/- 9.30%) (P < 0.005 and P < 0.05). MtDNA content correlated with function in mothers and infants. Maternofoetal parameters correlated at genetic and functional levels.Conclusion: HAART toxicity caused mitochondrial damage in HIV-infected pregnant women and their newborns, being present at a genetic and functional level with a maternofoetal correlation. (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins

Mitochondrial disturbances in HIV pregnancies

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