Isolation and characterization of H7N9 viruses from live poultry markets-Implication of the source of current H7N9 infection in humans
Isolation and characterization of H7N9 viruses from live poultry markets-Implication of the source of current H7N9 infection in humans
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DOI：
10.1007/s11434-013-5873-4
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发表时间：
2013-06-01
影响因子：
--
通讯作者：
Chen HuaLan
中科院分区：
文献类型：
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作者：
Shi JianZhong;Deng GuoHua;Chen HuaLan
2013年3月31日，国家卫生和计划生育委员会宣布，中国上海和安徽省发生了人感染甲型H7N9流感病毒的病例。为了调查导致这些人类感染的H7N9病毒的可能来源，我们从上海和安徽省的活禽市场和家禽养殖场收集了970份样品，包括饮用水，土壤，以及家禽的泄殖腔和气管拭子。20份样本对H7N9流感病毒呈阳性。值得注意的是，所有20种病毒都是从上海活禽市场收集的样本中分离出来的。系统进化分析表明，这些新的人H7N9病毒的6个内部基因来自禽H9N2病毒，但其HA和NA基因的祖先是不确定的。当我们检查活禽市场的H7N9分离株与引起人类感染的病毒之间的系统发育关系时，我们发现它们在所有八个基因片段上都具有高度同源性。因此，我们确定了导致人类感染的H7N9流感病毒的直接禽源。重要的是，我们观察到从人类分离的H7N9病毒已经获得了关键突变，使它们更像人类。因此，必须采取强有力的措施控制H7N9病毒在鸟类和人类中的传播，以防止对人类健康的进一步威胁。
On March 31, 2013, the National Health and Family Planning Commission announced that human infections with a previously undescribed influenza A (H7N9) virus had occurred in Shanghai and Anhui Province, China. To investigate the possible origins of the H7N9 viruses causing these human infections, we collected 970 samples, including drinking water, soil, and cloacal and tracheal swabs of poultry from live poultry markets and poultry farms in Shanghai and Anhui Province. Twenty samples were positive for the H7N9 influenza virus. Notably, all 20 viruses were isolated from samples collected from live poultry markets in Shanghai. Phylogenetic analyses showed that the six internal genes of these novel human H7N9 viruses were derived from avian H9N2 viruses, but the ancestor of their HA and NA genes is uncertain. When we examined the phylogenetic relationship between the H7N9 isolates from live poultry markets and the viruses that caused the human infections, we found that they shared high homology across all eight gene segments. We thus identified the direct avian origin of the H7N9 influenza viruses that caused the human infections. Importantly, we observed that the H7N9 viruses isolated from humans had acquired critical mutations that made them more "human-like". It is therefore imperative to take strong measures to control the spread of H7N9 viruses in birds and humans to prevent further threats to human health.