Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays
Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays
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DOI：
10.1073/pnas.041605498
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发表时间：
2001-02-27
影响因子：
11.1
通讯作者：
Hayashizaki, Y
中科院分区：
文献类型：
--
作者：
Miki, R;Kadota, K;Hayashizaki, Y
我们利用含有18，816个小鼠cDNA的cDNA微阵列系统地表征了49个成年和胚胎小鼠组织中的基因表达模式。聚类分析定义了广泛表达或在消化器官和肌肉等相似组织中表达的基因组。在胚胎脑、出生后小脑和成年嗅球中观察到表达谱的聚类，反映了神经发生和重塑的相似性。最后，将编码已知酶的基因聚类到78个代谢途径中，揭示了不同组织中每个途径内表达的惊人协调。另一方面，对糖酵解的更详细的检查揭示了关键调节酶谱的组织特异性差异。因此，通过使用具有大量元素的微阵列来调查全球基因表达，我们提供了对负责哺乳动物身体计划的发展和维持的途径的共性和多样性的见解。
We have systematically characterized gene expression patterns in 49 adult and embryonic mouse tissues by using cDNA microarrays with 18,816 mouse cDNAs. Cluster analysis defined sets of genes that were expressed ubiquitously or in similar groups of tissues such as digestive organs and muscle. Clustering of expression profiles was observed in embryonic brain, postnatal cerebellum, and adult olfactory bulb, reflecting similarities in neurogenesis and remodeling. Finally, clustering genes coding for known enzymes into 78 metabolic pathways revealed a surprising coordination of expression within each pathway among different tissues. On the other hand, a more detailed examination of glycolysis revealed tissue-specific differences in profiles of key regulatory enzymes. Thus, by surveying global gene expression by using microarrays with a large number of elements, we provide insights into the commonality and diversity of pathways responsible for the development and maintenance of the mammalian body plan.