STE20-LIKE PROTEIN-KINASES ARE REQUIRED FOR NORMAL LOCALIZATION OF CELL-GROWTH AND FOR CYTOKINESIS IN BUDDING YEAST
STE20-LIKE PROTEIN-KINASES ARE REQUIRED FOR NORMAL LOCALIZATION OF CELL-GROWTH AND FOR CYTOKINESIS IN BUDDING YEAST
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DOI：
10.1101/gad.9.15.1817
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发表时间：
1995-08-01
影响因子：
10.5
通讯作者：
NASMYTH, K
中科院分区：
文献类型：
--
作者：
CVRCKOVA, F;DEVIRGILIO, C;NASMYTH, K
酵母 Ste20 蛋白激酶参与信息素反应。 Ste20 的哺乳动物同源物存在，但其功能仍然未知。我们在缺乏 G(1) 细胞周期蛋白 Cln1 和 Cln2 的情况下筛选致死突变，鉴定出一种新型酵母 STE20 同源物 CLA4。 Cla4 参与出芽和胞质分裂，并与 Cdc42（极化细胞生长所需的 GTP 酶）相互作用。尽管存在胞质分裂缺陷，cla4 突变体仍然具有活力。然而，双 cla4 ste20 突变体不能在芽颈处维持隔膜环，并且不能进行胞质分裂。在同一筛选中发现了编码其中一种脓毒蛋白的 CDC12 突变，Cla4 和 Ste20 激酶显然在相对于脓毒蛋白环定位细胞生长方面具有共同的功能。
The yeast Ste20 protein kinase is involved in pheromone response. Mammalian homologs of Ste20 exist, but their function remains unknown. We identified a novel yeast STE20 homolog, CLA4, in a screen for mutations lethal in the absence of the G(1) cyclins Cln1 and Cln2. Cla4 is involved in budding and cytokinesis and interacts with Cdc42, a GTPase required for polarized cell growth. Despite a cytokinesis defect, cla4 mutants are viable. However, double cla4 ste20 mutants cannot maintain septin rings at the bud neck and cannot undergo cytokinesis. Mutations in CDC12, which encodes one of the septins, were found in the same screen, Cla4 and Ste20 kinases apparently share a function in localizing cell growth with respect to the septin ring.