Rat adipose tissue cAMP-dependent protein kinase: a unique form of type II
Rat adipose tissue cAMP-dependent protein kinase: a unique form of type II
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大鼠脂肪组织 cAMP 依赖性蛋白激酶：II 型的独特形式
DOI：
10.1016/0303-7207(84)90086-8
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发表时间：
1984
影响因子：
4.1
通讯作者：
J. Corbin
中科院分区：
文献类型：
--
作者：
S. Beebe;J. Corbin
The rat adipose tissue cAMP-dependent protein kinase type II holoenzyme and regulatory (R) subunit were compared with type II from bovine heart and several other species and tissues. Adipose tissue type II was similar to the bovine heart type II by several criteria (S20,w= 7.0, site 1 and site 2 dissociation rates for [3H]cAMP, rapid autophosphorylation and lack of MgATP inhibition of [3H]cAMP binding). However, some of its physical characteristics were similar to type I. The apparent molecular weight determined by SDS gel electrophoresis of the homogeneous adipose tissue R subunit was 51 000 daltons compared to 49000 for type I and 53000–58000 for other type II R subunits. The adipose tissue holoenzyme eluted from DEAE-cellulose at an intermediate position between type I and bovine heart type II. The adipose tissue and bovine heart holoenzymes differed in several properties including Stokes radius (5.2 nm vs. 6.0 nm), calculated molecular weight (157000 vs. 181000 daltons) and frictional ratio (1.47 vs. 1.60). After autophosphorylation the adipose tissue R subunit, like type IIB forms from other species and tissues, did not shift to a higher apparenl molecular weight on SDS gel electrophoresis like bovine heart type IIR subunit (a type IIA form). Even though the adipose tissue enzyme was quite similar to other type II forms in the kinetics of cAMP action, the cAMP binding sites could be shown lo be differenl from them by the use of cAMP analogs. cAMP analogs modified at the N6position of the adenine ring, such asN6-benzoyl cAMP, had higher apparentKavalues for protein kinase activation for the adipose tissue enzyme than for the bovine and several other heart isozymes. cAMP analogs modified at the 8 carbon of the adenine ring showed positive cooperativity of activation for the adipose tissue enzyme but not for the bovine heart holoenzyme. The adipose lissue isozyme is the first type II form described to have a distinct kinetic characteristic.
环核苷酸类似物对蛋白激酶同工酶链内位点 I 的影响。
DOI：
10.1111/j.1432-1033.1982.tb06677.x
发表时间：
1982
期刊：
European journal of biochemistry
影响因子：
--
作者：
Corbin,JD;Rannels,SR;Flockhart,DA;Robinson-Steiner,AM;Tigani,MC;Døskeland,SO;Suva,RH;Suva,R;Miller,JP
通讯作者：
Miller,JP
两个链内 cAMP 结合位点可能参与蛋白激酶的激活。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Robinson-Steiner,AM;Corbin,JD
通讯作者：
Corbin,JD
II 型 cAMP 依赖性蛋白激酶的两个亚类的鉴定。
DOI：
--
发表时间：
1980
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Erlichman,J;Sarkar,D;Fleischer,N;Rubin,CS
通讯作者：
Rubin,CS