Modeling studies of cell response to ultrashort, high-intensity electric fields-implications for intracellular manipulation
Modeling studies of cell response to ultrashort, high-intensity electric fields-implications for intracellular manipulation
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DOI：
10.1109/tps.2004.830971
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发表时间：
2004-08-01
影响因子：
1.5
通讯作者：
Schoenbach, KH
中科院分区：
文献类型：
--
作者：
Joshi, RP;Hu, Q;Schoenbach, KH
基于电流连续性和Smoluchowski方程的耦合模型，研究了纳秒级高强度脉冲作用下生物细胞电穿孔的动力学过程。提出了一种新的分布式网络模型，该模型包括细胞膜及其子结构的动态电导率，用于跨膜电位的计算。结果表明，亚细胞结构可以通过纳秒脉冲的影响，而且，尽管高场强，该过程仍然是非热的。作为一个例子的选择性，正常和恶性（法拉奇）扁桃体B细胞之间的细胞反应的差异进行了比较和讨论。研究表明，超短、高强度的电脉冲可以损伤癌细胞。最后，该模型预测有可能靶向线粒体内膜（即，细胞器水平的选择性），与最近的实验观察一致。
The dynamics of electroporation in biological cells subjected to nanosecond, high-intensity pulses are studied based on a coupled scheme involving both the current continuity and Smoluchowski equations. A new distributed network model, that includes dynamic conductivities of cell membranes and substructures, is introduced for evaluations of transmembrane potential. It is shown that subcellular structures could be affected through nanosecond pulses, and that, despite the high field intensity, the processes remain nonthermal. As an example of selectivity, differences in cell responses between normal and malignant (Farage) tonsillar B-cells are compared and discussed. It is shown that ultrashort, high-intensity electric pulses could damage cancer cells. Finally, the model predicts that it is possible to target the inner mitochondrial membrane (i.e., selectivity at the organelle level), in keeping with recent experimental observations.