The Number of Natural Killer Cells in the Largest Diameter Lymph Nodes Is Associated with the Number of Retrieved Lymph Nodes and Lymph Node Size, and Is an Independent Prognostic Factor in Patients with Stage II Colon Cancer
The Number of Natural Killer Cells in the Largest Diameter Lymph Nodes Is Associated with the Number of Retrieved Lymph Nodes and Lymph Node Size, and Is an Independent Prognostic Factor in Patients with Stage II Colon Cancer
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DOI：
10.1159/000491019
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发表时间：
2018-01-01
期刊：
影响因子：
3.5
通讯作者：
Suzuki, Toshiyuki
中科院分区：
文献类型：
--
作者：
Okada, Kazutake;Sadahiro, Sotaro;Suzuki, Toshiyuki
目的：我们以前曾报道，最大直径的回收淋巴结（LN）与LN的数量相关，是一个预后因素，在第二阶段结肠癌。我们研究淋巴结中的T、B和自然杀伤（NK）细胞是否与淋巴结的数量和存活率相关。方法：研究对象包括320例II期结肠癌患者。在每例患者中选择具有最大直径的LN。测量CD 3和CD 20染色的细胞的阳性面积比以及CD 56阳性细胞的数量。结果：CD 3阳性面积比为0.39 +/- 0.08，CD 20阳性面积比为0.42 +/- 0.10。CD 56阳性细胞的平均数量为19.3 +/- 22.7。淋巴结最大直径与B细胞、T细胞面积比及NK细胞数量呈显著正相关。NK细胞数量与淋巴结数量显著相关，是独立的预后因素。多变量分析发现，病理T分期（T4或T3; HR 4.71; p < 0.001）和CD 56阳性细胞数（高或低; HR 0.22; p < 0.001）是独立的预后因素。结论：最大直径淋巴结中NK细胞的数量最有可能被用作复发的预测因子。
Objective: We previously reported that the largest diameter of retrieved lymph nodes (LNs) correlates with the number of LNs and is a prognostic factor in stage II colon cancer. We examine whether T, B, and natural killer (NK) cells in LNs are related to the number of LNs and survival. Methods: The subjects comprised 320 patients with stage II colon cancer. An LN with the largest diameter was selected in each patient. The positive area ratios of cells that stained for CD3 and CD20, and the numbers of CD56-positive cells were measured. Results: The CD3-positive area ratio was 0.39 +/- 0.08 and CD20-positive area ratio was 0.42 +/- 0.10. The mean number of CD56-positive cells was 19.3 +/- 22.7. The area ratios of B cells and T cells and the number of NK cells were significantly related to the sizes of the largest diameter LNs. The number of NK cells significantly correlated with the number of LNs and was an independent prognostic factor. On multivariate analysis, pathological T stage (T4 or T3; HR 4.71; p < 0.001) and the number of CD56-positive cells (high or low; HR 0.22; p < 0.001) were found to be independent prognostic factors. Conclusions: The number of NK cells in the largest diameter LNs can most likely be used as a predictor of recurrence.