The microtubule network of renal epithelial cells is disrupted by ischemia and reperfusion.
The microtubule network of renal epithelial cells is disrupted by ischemia and reperfusion.
复制标题
肾上皮细胞的微管网络因缺血和再灌注而被破坏。
DOI：
10.1152/ajprenal.1994.267.6.f971
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Brown,D
中科院分区：
文献类型：
--
作者：
Abbate,M;Bonventre,JV;Brown,D
缺血导致肾上皮细胞质膜完整性的改变、细胞极性的改变和细胞分裂的开始。由于微管参与了这些过程，我们研究了缺血和再灌注对大鼠肾脏微管细胞骨架的影响。在缺血40分钟和再灌注1小时后检测到S3近端小管微管网络的主要变化。微管断裂，抗微管抗体染色明显低于正常肾脏。靠近髓质维管束的Henle升肢部分厚，微管蛋白染色变化性丧失。再灌注24和48小时后，与基底膜接触的大多数近端小管细胞中再次出现微管蛋白标记，但在脱落细胞中未检测到微管蛋白标记。再灌注48小时后，肾脏出现大量有丝分裂现象。缺血40分钟无再灌注的肾脏和再灌注1小时后的对侧肾脏仅显示轻度微管破坏。由于微管在上皮细胞极性的产生和维持中发挥了既定的作用，它们的丢失可能导致缺血和再灌注后发生的结构变化。
Ischemia results in alterations in the integrity of the plasma membrane of renal epithelial cells, changes in cell polarity, and initiation of cell division. Because microtubules are implicated in these processes, we examined the effects of ischemia and reperfusion on the microtubular cytoskeleton in rat kidney. Major alterations in the microtubule network of S3 proximal tubules were detected after 40 min of ischemia followed by 1 h of reperfusion. There was fragmentation of microtubules and considerably less intense staining with antitubulin antibodies than with normal kidneys. Some thick ascending limbs of Henle close to medullary vascular bundles showed a variable loss of tubulin staining. After 24 and 48 h of reperfusion, tubulin labeling was again present in most proximal tubule cells in contact with the basement membrane but was not detectable in exfoliated cells. Numerous mitotic figures were present in kidneys 48 h after reperfusion. Kidneys subjected to 40 min of ischemia without reperfusion and contralateral kidneys studied after 1 h of reperfusion showed only mild microtubular disruption. Because of the established role of microtubules in the generation and maintenance of epithelial cell polarity, their loss may contribute to structural changes that occur after ischemia and reperfusion.