Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant Enzyme Activities in Kidney Tubular Epithelial Cell Line, LLC-PK1
Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant Enzyme Activities in Kidney Tubular Epithelial Cell Line, LLC-PK1
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DOI：
10.1111/bcpt.12110
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发表时间：
2013-12-01
影响因子：
3.1
通讯作者：
Sun, Zhi-Liang
中科院分区：
文献类型：
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作者：
Yu, Chun-Hong;Liu, Zhao-Ying;Sun, Zhi-Liang
本研究旨在探讨氧化应激参与丹诺沙星对肾小管上皮细胞系(LLC-PK1)的毒性作用。将融合的LLC-PK1细胞与不同浓度的丹诺沙星孵育。通过检测细胞内活性氧(ROS)水平、脂质过氧化反应、细胞凋亡和抗氧化酶活性来评价氧化损伤程度。丹诺沙星诱导的ROS产生呈浓度依赖性增加，甚至在细胞毒性条件下也不能。同样，丹诺沙星在400M作用24小时后，可使硫代巴比妥酸活性物质的含量增加约4倍，但不能诱导细胞毒性和细胞凋亡。100、200和400M的丹诺沙星处理24小时后，超氧化物歧化酶(SOD)和过氧化氢酶(CAT)等抗氧化酶活性均升高。谷胱甘肽过氧化物酶(GPX)活性显著降低，且呈浓度依赖性。此外，添加谷胱甘肽和N-乙酰半胱氨酸可抑制ROS的产生、脂质过氧化和GPx的下降。这些结果表明，丹诺沙星在400M浓度作用24小时不能诱导LLC-PK1细胞产生氧化应激。超氧化物歧化酶和过氧化氢酶活性的升高可部分缓解体内ROS水平和脂质过氧化水平的升高。
The purpose of this study was to investigate the possibility that oxidative stress was involved in danofloxacin-induced toxicity in renal tubular cells epithelial cell line (LLC-PK1). Confluent LLC-PK1 cells were incubated with various concentrations of danofloxacin. The extent of oxidative damage was assessed by measuring the reactive oxygen species (ROS) level, lipid peroxidation, cell apoptosis and antioxidative enzyme activities. Danofloxacin induced a concentration-dependent increase in the ROS production, not even cytotoxic conditions. Similarly, danofloxacin caused an about 4 times increase in the level of thiobarbituric acid reactive substances at the concentration of 400M for 24hr, but it did not induce cytotoxicity and apoptosis. Antioxidant enzymes activities, such as superoxide dismutase (SOD) and catalase (CAT), were increased after treatment with 100, 200 and 400M of danofloxacin for 24hr. The activity of glutathione peroxidase (GPX) was significantly decreased in a concentration-dependent manner. In addition, ROS production, lipid peroxidation and GPX decline were inhibited by additional glutathione and N-acetyl cysteine. These data suggested that danofloxacin could not induce oxidative stress in LLC-PK1 cells at the concentration (400M) for 24hr. The increase levels of ROS and lipid peroxidation could be partly abated by the increase activities of SOD and CAT.