A MEIOSIS-SPECIFIC PROTEIN-KINASE HOMOLOG REQUIRED FOR CHROMOSOME SYNAPSIS AND RECOMBINATION
A MEIOSIS-SPECIFIC PROTEIN-KINASE HOMOLOG REQUIRED FOR CHROMOSOME SYNAPSIS AND RECOMBINATION
复制标题
DOI：
10.1101/gad.5.12b.2392
复制
发表时间：
1991-12-01
影响因子：
10.5
通讯作者：
ROEDER, GS
中科院分区：
文献类型：
--
作者：
ROCKMILL, B;ROEDER, GS
从酿酒酵母中分离出mek1（减数分裂激酶）突变体。mek1零突变的二倍体纯合子仅产生13%的活孢子。mek1孢子的失活性由spo13突变挽救，这导致细胞绕过减数分裂I分裂。在mek1零突变体中，减数分裂重组减少，但没有完全消除。mek1二倍体减数分裂染色体的细胞核扩散显示出比野生型SC更短的突触复合体（SC）的许多延伸。mek1::1acZ融合基因的分析和Northern blot杂交表明，mek1转录物仅存在于减数分裂中。MEK1基因序列预测了一个与丝氨酸-苏氨酸蛋白激酶同源的56.8 kd蛋白。MEK1基因定位在CDC64近端13 cM的XV染色体上。提出了MEK1基因产物的功能模型。
The mek1 (meiotic kinase) mutant of Saccharomyces cerevisiae was isolated in a screen for sporulation-proficient, meiotic-lethal mutants. Diploids homozygous for a mek1 null mutation produce only 13% viable spores. mek1 spore inviability is rescued by a spo13 mutation, which causes cells to bypass the meiosis I division. In a mek1 null mutant, meiotic recombination is reduced but not completely eliminated. Nuclear spreads of meiotic chromosomes from mek1 diploids reveal numerous stretches of synaptonemal complex (SC) that are shorter than wild-type SCs. Analysis of a mek1::1acZ fusion gene and Northern blot hybridization demonstrate that the MEK1 transcript is present only in meiosis. The sequence of the MEK1 gene predicts a 56.8-kD protein with homology to serine-threonine protein kinases. The MEK1 gene maps to chromosome XV, 13 cM proximal to CDC64. Models for the function of the MEK1 gene product are proposed.