A regulon conserved in monocot and dicot plants defines a functional module in antifungal plant immunity
A regulon conserved in monocot and dicot plants defines a functional module in antifungal plant immunity
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DOI：
10.1073/pnas.1003619107
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发表时间：
2010-12-14
影响因子：
11.1
通讯作者：
Panstruga, Ralph
中科院分区：
文献类型：
--
作者：
Humphry, Matt;Bednarek, Pawel;Panstruga, Ralph
调节植物对真菌性白粉病的抗性的至少两种组分是古老的，并且自单子叶-双子叶植物分裂（大约200 Mya）以来一直被保存。这些组分是含有MLO/MLO 2蛋白和突触融合蛋白ROR 2/PEN 1的七个跨膜结构域，它们具有拮抗作用，并且已经分别在单子叶大麦（Hordeum vulgare）和双子叶拟南芥中鉴定。此外，突触融合蛋白相互作用的N-乙基马来酰亚胺敏感因子衔接蛋白受体蛋白（VAMP 721/722和SNAP 33/34）以及黑芥子酶（PEN 2）和ABC转运蛋白（PEN 3）有助于大麦和/或拟南芥中的抗真菌抗性。在这里，我们表明，这些遗传定义的防御组件共享一组相似的共表达基因在两种植物物种，包括一个统计学上显着的过度表达的基因产物参与调控的转录，翻译后修饰，和信号。大多数共表达的拟南芥基因具有共同的顺式调控元件，可能决定其协调表达。我们利用基因共表达来揭示拟南芥中参与抗真菌防御的许多组分。总之，我们的数据提供了进化保守的调节子组成的核心组件和进化枝/物种特异性的创新，作为一个模块在植物先天免疫功能的证据。
At least two components that modulate plant resistance against the fungal powdery mildew disease are ancient and have been conserved since the time of the monocot-dicot split (approximate to 200 Mya). These components are the seven transmembrane domain containing MLO/MLO2 protein and the syntaxin ROR2/PEN1, which act antagonistically and have been identified in the monocot barley (Hordeum vulgare) and the dicot Arabidopsis thaliana, respectively. Additionally, syntaxin-interacting N-ethylmaleimide sensitive factor adaptor protein receptor proteins (VAMP721/722 and SNAP33/34) as well as a myrosinase (PEN2) and an ABC transporter (PEN3) contribute to antifungal resistance in both barley and/or Arabidopsis. Here, we show that these genetically defined defense components share a similar set of coexpressed genes in the two plant species, comprising a statistically significant overrepresentation of gene products involved in regulation of transcription, post-translational modification, and signaling. Most of the coexpressed Arabidopsis genes possess a common cis-regulatory element that may dictate their coordinated expression. We exploited gene coexpression to uncover numerous components in Arabidopsis involved in antifungal defense. Together, our data provide evidence for an evolutionarily conserved regulon composed of core components and clade/species-specific innovations that functions as a module in plant innate immunity.