Comparison of metabolite profiles in U87 glioma cells and mesenchymal stem cells
Comparison of metabolite profiles in U87 glioma cells and mesenchymal stem cells
复制标题
DOI：
10.1016/j.biosystems.2011.05.005
复制
发表时间：
2011-08-01
期刊：
影响因子：
1.6
通讯作者：
Selbig, Joachim
中科院分区：
文献类型：
--
作者：
Juerchott, Kathrin;Guo, Ke-Tai;Selbig, Joachim
从U87胶质瘤细胞和人间充质干细胞（hMSC）产生气相色谱-质谱（GC-MS）谱。选择代表糖酵解中间体、TCA循环代谢物、氨基酸和脂质的37种代谢物进行详细分析。这些的浓度。比较代谢物，并使用Pearson相关系数计算代谢物对之间的关系。两种细胞系之间的代谢产物谱和相关模式显著不同。这些特征可以被认为是潜在的生化系统的特征，并提供了间充质干细胞和肿瘤细胞中代谢的快照。(C)2011爱思唯尔爱尔兰有限公司保留所有权利。
Gas chromatography-mass spectrometry (GC-MS) profiles were generated from U87 glioma cells and human mesenchymal stem cells (hMSC). 37 metabolites representing glycolysis intermediates, TCA cycle metabolites, amino acids and lipids were selected for a detailed analysis. The concentrations of these. metabolites were compared and Pearson correlation coefficients were used to calculate the relationship between pairs of metabolites. Metabolite profiles and correlation patterns differ significantly between the two cell lines. These profiles can be considered as a signature of the underlying biochemical system and provide snap-shots of the metabolism in mesenchymal stem cells and tumor cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.