Expression and cellular localization of classic NADPH oxidase subunits in the spontaneously hypertensive rat kidney
Expression and cellular localization of classic NADPH oxidase subunits in the spontaneously hypertensive rat kidney
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DOI：
10.1161/hy0202.103264
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发表时间：
2002-02-01
期刊：
影响因子：
8.3
通讯作者：
Wilcox, CS
中科院分区：
文献类型：
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作者：
Chabrashvili, T;Tojo, A;Wilcox, CS
吞噬细胞通过一种经典的由5个组分构成的NADPH氧化酶产生超氧阴离子（O - 2（ - ））。O - 2（ - ）在自发性高血压大鼠（SHR）中会导致高血压。因此，我们检验了在SHR肾脏中NADPH氧化酶表达增强这一假设。我们还分析了NADPH氧化酶组分在SHR肾脏中的定位。通过逆转录 - 聚合酶链反应和蛋白质印迹法对肾脏NADPH氧化酶进行定量，并通过免疫组织化学方法对其在SHR和京都威斯塔大鼠（WKY）肾脏中的定位进行研究。在成年大鼠肾脏中检测到了吞噬细胞NADPH氧化酶5个亚基以及MOX1和RENOX（NOX4）的mRNA。成年（10周龄）SHR的肾脏具有显著的（P<......此处原文似乎不完整）
Phagocytes generate superoxide anion (O-2(-)) by a classic, 5-component NADPH oxidase. O-2(-) contributes to hypertension in spontaneously hypertensive rats (SHR). Therefore, we tested the hypothesis that NADPH oxidase expression is enhanced in the SHR kidney. We also analyzed the localization of NADPH oxidase components in SHR kidney. Renal NADPH oxidase was quantified by reverse transcription-polymerise chain reaction and Western blotting and was localized in SHR and Wistar Kyoto rat (WKY) kidney by immunohistochemistry. The mRNA for 5 subunits of phagocyte NADPH oxidase, and also for MOX1 and RENOX (NOX4), was detected in adult rat kidney. Kidneys of adult (10 weeks old) SHR had a significantly (P