Evidence for abnormal Na+/H+ antiport activity detected by phosphorus nuclear magnetic resonance spectroscopy in exercising skeletal muscle of patients with essential hypertension.
Evidence for abnormal Na+/H+ antiport activity detected by phosphorus nuclear magnetic resonance spectroscopy in exercising skeletal muscle of patients with essential hypertension.
复制标题
通过磷核磁共振波谱检测原发性高血压患者骨骼肌运动中 Na /H 逆向转运活性异常的证据。
DOI：
10.1042/cs0790491
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发表时间：
1990
期刊：
影响因子：
6
通讯作者：
J. Ledingham
中科院分区：
文献类型：
--
作者：
C. Dudley;D. Taylor;L. Ng;G. Kemp;P. Ratcliffe;G. Radda;J. Ledingham
1.本文应用~（31）P核磁共振技术研究了8例原发性高血压患者运动诱发的骨骼肌pH值变化。谱2.白细胞Na+/H+反向转运活性在体外测定相同的科目使用pH敏感的荧光染料。3.静息骨骼肌pH值和未受刺激的白细胞pH值与对照组相似，但高血压患者的白细胞通过体外酸负荷表现出Na+/H+逆向转运活性增加。在这些相同的患者中，在等张运动诱导的酸负荷期间，体内也证明了骨骼肌酸化减少和pH恢复速率增加。4.这些研究结果表明，增加细胞Na+/H+逆向转运活动，这已被证明在体外原发性高血压，也影响骨骼肌生理水平的运动的生化反应。这加强了高血压中Na+/H+反向转运活性增加是一种普遍的生理相关细胞异常的论点。
1. Exercise-induced pH changes in skeletal muscle were studied in a group of eight subjects with essential hypertension by using 31P n.m.r. spectroscopy. 2. Leucocyte Na+/H+ antiport activity was measured in vitro in the same subjects using a pH-sensitive fluorescent dye. 3. Resting skeletal muscle pH and unstimulated leucocyte pH values were similar to those in control subjects, but increased Na+/H+ antiport activity was demonstrated in the leucocytes from hypertensive subjects by acid loading in vitro. Decreased skeletal muscle acidification and an increased rate of pH recovery was also demonstrated in vivo in these same patients during an acid load induced by isotonic exercise. 4. These findings suggest that the increased cellular Na+/H+ antiport activity, which has been demonstrated in vitro in essential hypertension, also affects the biochemical response of skeletal muscle to physiological levels of exercise. This strengthens the argument that increased Na+/H+ antiport activity in hypertension is a generalized and physiologically relevant cellular abnormality.