The Role of cGMP-Dependent Signaling Pathway in Synaptic Vesicle Cycle at the Frog Motor Nerve Terminals
The Role of cGMP-Dependent Signaling Pathway in Synaptic Vesicle Cycle at the Frog Motor Nerve Terminals
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DOI：
10.1523/jneurosci.2947-08.2008
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发表时间：
2008-12-03
影响因子：
5.3
通讯作者：
Zefirov, Andrej L.
中科院分区：
文献类型：
--
作者：
Petrov, Aleksej M.;Giniatullin, Arthur R.;Zefirov, Andrej L.
利用电生理和荧光方法研究了长时间高频刺激下cgmp依赖性通路在运动神经末梢突触囊泡循环中的作用。细胞内cGMP浓度（8-Br-cGMP或8-pCPT-cGMP）的增加，通过提高从回收池到易释放池的囊泡交通速率和加速快速内吞作用，显著缩短突触囊泡的循环时间。可溶性鸟苷酸环化酶或蛋白激酶G的药理抑制减慢了突触囊泡的循环速率和内吞作用。提示cgmp - pkg依赖通路在蛙运动末端的水泡周期调控中起重要作用。
The role of cGMP-dependent pathways in synaptic vesicle recycling in motor nerve endings during prolonged high-frequency stimulation was studied at frog neuromuscular junctions using electrophysiological and fluorescent methods. An increase of intracellular cGMP concentration (8-Br-cGMP or 8-pCPT-cGMP) significantly reduced the cycle time for synaptic vesicles through the enhancement of vesicular traffic rate from the recycling pool to the readily releasable pool and acceleration of fast endocytosis. Pharmacological inhibition of soluble guanylate cyclase or protein kinase G slowed down the rate of recycling as well as endocytosis of synaptic vesicles. The results suggest that cGMP-PKG-dependent pathway serves a significant function in the control of vesicular cycle in frog motor terminals.