DETECTION OF EXOCYTOSIS AT INDIVIDUAL PANCREATIC BETA-CELLS BY AMPEROMETRY AT A CHEMICALLY-MODIFIED MICROELECTRODE
DETECTION OF EXOCYTOSIS AT INDIVIDUAL PANCREATIC BETA-CELLS BY AMPEROMETRY AT A CHEMICALLY-MODIFIED MICROELECTRODE
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DOI：
10.1073/pnas.92.21.9608
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发表时间：
1995-10-10
影响因子：
11.1
通讯作者：
KENNEDY, RT
中科院分区：
文献类型：
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作者：
HUANG, L;SHEN, H;KENNEDY, RT
用氧化钌和氰钌酸盐复合物修饰的碳纤维微电极上的安培法用于监测大鼠和人类单个胰腺β细胞的化学分泌物。当将胰岛素促分泌剂葡萄糖、甲苯磺丁脲和K+施加到细胞时，观察到一系列随机发生的电流尖峰。显示电流尖峰是由于检测到从细胞分泌的化学物质，色谱显示由电极检测到的主要分泌物质是胰岛素。电流尖峰强烈依赖于外部Ca2+，具有独立于刺激方法的平均面积，并且具有对应于β细胞中囊泡大小分布的面积分布。可以得出结论，尖峰是由于检测到由胞吐分泌的胰岛素的浓度脉冲。
Amperometry at a carbon fiber microelectrode modified with a composite of ruthenium oxide and cyanoruthenate was used to monitor chemical secretions of single pancreatic beta cells from rats and humans. When the insulin secretagogues glucose, tolbutamide, and K+ were applied to the cell, a series of randomly occurring current spikes was observed. The current spikes were shown to be due to the detection of chemical substances secreted from the cell, Chromatography showed that the primary secreted substance detected by the electrode was insulin. The current spikes were strongly dependent on external Ca2+, had an average area that was independent of the stimulation method, and had an area distribution which corresponded to the distribution of vesicle sizes in beta cells. It was concluded that the spikes were due to the detection of concentration pulses of insulin secreted by exocytosis.