LYSIS OF LYSIS-INHIBITED BACTERIOPHAGE T4-INFECTED CELLS
LYSIS OF LYSIS-INHIBITED BACTERIOPHAGE T4-INFECTED CELLS
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DOI：
10.1128/jb.174.24.8073-8080.1992
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发表时间：
1992-12-01
影响因子：
3.2
通讯作者：
ABEDON, ST
中科院分区：
文献类型：
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作者：
ABEDON, ST
T4噬菌体（噬菌体）感染的细胞在吸附额外的T4噬菌体（二次吸附）后，潜伏期长度显着增加，称为裂解抑制。裂解抑制是一种复杂的表型，需要至少6个T4基因的活性。两个基本的谜团围绕着我们对裂解抑制表达的理解：(i)起始机制（即，二次吸附如何导致裂解抑制表达）和（ii）裂解机制（即，不裂解的信号如何被逆转）。本研究首先涵盖了高培养密度下t4感染细胞裂解抑制和裂解表达的基本生物学。然后，证据表明，随着裂解抑制的开始，这些培养物的裂解相关的突然清除可能是由T4二次吸附引起的。例如，在T4原液制备过程中，在批量培养中生长的裂解抑制的T4感染细胞经常观察到这种清除。讨论了这种二次吸附诱导裂解对野生T4群体的意义。该研究得出了一个合乎逻辑的论点，表明T4噬菌体颗粒的裂解性质是噬菌体诱导裂解的一种新机制。
T4 bacteriophage (phage)-infected cells show a marked increase in latent-period length, called lysis inhibition, upon adsorption of additional T4 phages (secondary adsorption). Lysis inhibition is a complex phenotype requiring the activity of at least six T4 genes. Two basic mysteries surround our understanding of the expression of lysis inhibition: (i) the mechanism of initiation (i.e., how secondary adsorption leads to the expression of lysis inhibition) and (ii) the mechanism of lysis (i.e., how this signal not to lyse is reversed). This study first covers the basic biology of the expression of lysis inhibition and lysis of T4-infected cells at high culture densities. Then evidence is presented which implies that, as with the initiation of lysis inhibition, sudden, lysis-associated clearing of these cultures is likely caused by T4 secondary adsorption. For example, such clearing is often observed for lysis-inhibited T4-infected cells grown in batch culture during T4 stock preparation. The significance of this secondary adsorption-induced lysis to wild T4 populations is discussed. The study concludes with a logical argument suggesting that the lytic nature of the T4 phage particle evolved as a novel mechanism of phage-induced lysis.