The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice
The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice
复制标题
三螺旋转录因子 OsGT gamma-2 参与水稻对盐胁迫的适应
DOI：
10.1007/s11103-020-01010-1
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发表时间：
2020-06-05
影响因子：
5.1
通讯作者：
Li, Juan
中科院分区：
文献类型：
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作者：
Liu, Xiaoshuang;Wu, Dechuan;Li, Juan
关键信息 OsGT gamma-2 是一种三螺旋转录因子，通过调节离子转运蛋白的表达，是水稻对盐胁迫反应的正调节因子。盐胁迫严重制约水稻的生长和产量。 Trihelix 转录因子（GT 因子）与 GT 元件特异性结合，并在植物形态发育和对非生物胁迫的响应中发挥多种作用。在我们之前的研究中，我们发现GT-1元件（GAAAAA）是盐度诱导的OsRAV2启动子中的关键元件。在这里，我们鉴定了水稻 OsGT gamma 家族成员 OsGT gamma-2，它直接与 OsRAV2 启动子中的 GT-1 元件相互作用。 OsGT gamma-2 特异性靶向细胞核，主要在根、鞘、茎和种子中表达，并由盐度、渗透和氧化应激以及脱落酸 (ABA) 诱导。 OsGT gamma-2 过表达株系（P-ZmUbi::OsGT gamma-2）的种子发芽率、幼苗生长和盐胁迫下的存活率均得到提高。相比之下，CRISPR/Cas9 介导的 OsGT gamma-2 敲除系 (osgt gamma-2) 显示出盐过敏表型。为了响应盐胁迫，在 P-ZmUbi::OsGT gamma-2 品系和 osgt gamma-2 植物中观察到不同的 Na+ 和 K+ 响应模式。还研究了 OsGT gamma-2 在水稻盐适应中的分子机制。几个负责离子转运的主要基因，如OsHKT2； 1、OsHKT1； 3 和 OsNHX1 受 OsGT gamma-2 转录调节。随后的酵母单杂交测定和 EMSA 表明 OsGT gamma-2 直接与 OsHKT2 的启动子相互作用； 1、OsNHX1和OsHKT1； 3. 综上所述，这些结果表明 OsGT gamma-2 是参与水稻对盐胁迫反应的重要正调节因子，并表明 OsGT gamma-2 在调节水稻盐度适应方面具有潜在作用。
Key message OsGT gamma-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role in plant morphological development and responses to abiotic stresses. In our previous study, we found that the GT-1 element (GAAAAA) is a key element in the salinity-induced OsRAV2 promoter. Here, we identified a rice OsGT gamma family member, OsGT gamma-2, which directly interacted with the GT-1 element in the OsRAV2 promoter. OsGT gamma-2 specifically targeted the nucleus, was mainly expressed in roots, sheathes, stems and seeds, and was induced by salinity, osmotic and oxidative stresses and abscisic acid (ABA). The seed germination rate, seedling growth and survival rate under salinity stress was improved in OsGT gamma-2 overexpressing lines (P-ZmUbi::OsGT gamma-2). In contrast, CRISPR/Cas9-mediated OsGT gamma-2 knockout lines (osgt gamma-2) showed salt-hypersensitive phenotypes. In response to salt stress, different Na+ and K+ acclamation patterns were observed in P-ZmUbi::OsGT gamma-2 lines and osgt gamma-2 plants were observed. The molecular mechanism of OsGT gamma-2 in rice salt adaptation was also investigated. Several major genes responsible for ion transporting, such as the OsHKT2; 1, OsHKT1; 3 and OsNHX1 were transcriptionally regulated by OsGT gamma-2. A subsequent yeast one-hybrid assay and EMSA indicated that OsGT gamma-2 directly interacted with the promoters of OsHKT2; 1, OsNHX1 and OsHKT1; 3. Taken together, these results suggest that OsGT gamma-2 is an important positive regulator involved in rice responses to salt stress and suggest a potential role for OsGT gamma-2 in regulating salinity adaptation in rice.