Whole Genomic Characterization of a Korean Human Parechovirus Type 1 (HPeV1) Identifies Recombination Events
Whole Genomic Characterization of a Korean Human Parechovirus Type 1 (HPeV1) Identifies Recombination Events
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DOI：
10.1002/jmv.23921
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发表时间：
2014-12-01
影响因子：
12.7
通讯作者：
Kim, Wonyong
中科院分区：
文献类型：
--
作者：
Truong Cong Thoi;Van Thai Than;Kim, Wonyong
使用宏基因组学方法从韩国一名2岁健康女婴的粪便样本中检测到人双埃柯病毒（HPeV）株HPE 10-NN。使用细胞培养系统分离了HPV 10-NN病毒，并对其全基因组进行了分析。该菌株的RNA基因组由7，348个核苷酸（nt）组成，不包括poly（A）尾。编码2，180个氨基酸的推定多聚蛋白前体的6，540 nt的大开放阅读框侧翼为708 nt的5-非翻译区（UTR）和88 nt的3-UTR，随后为poly（A）尾。系统进化分析表明，该毒株属于HPeV 1。SimPlot和Bootscan分析表明，病毒基因组由与其他HPeV相应基因组区域相关的区域组成。重组分析表明，HPV 10-NN毒株可能是HPeV 1、HPeV 3和HPeV 4毒株之间发生的一个以上基因组重组事件的产物。医学病毒学杂志86：2084-2091，2014. (c)2014 Wiley Periodicals，Inc.
A human parechovirus (HPeV) strain CAU10-NN was detected from a stool sample of a 2-year-old healthy female infant in South Korea using a metagenomic approach. The CAU10-NN virus was isolated using a cell culture system and its whole genome was analyzed. The RNA genome of the CAU10-NN strain consists of 7,348 nucleotides (nt), excluding a poly(A) tail. A large open reading frame of 6,540 nt that encodes a putative polyprotein precursor of 2,180 amino acids is flanked by a 5-untranslated region (UTR) of 708 nt and 3-UTR of 88 nt followed by a poly(A) tail. Phylogenetic analysis shows that the CAU10-NN strain belongs to HPeV1. SimPlot and Bootscan analyses reveal that the virus genome is composed of regions related to corresponding genomic regions of other HPeVs. Recombination analysis indicates that the CAU10-NN strain might be a product of more than one genomic recombination event that occurred among HPeV1, HPeV3, and HPeV4 strains. J. Med. Virol. 86:2084-2091, 2014. (c) 2014 Wiley Periodicals, Inc.