MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.
MiR-429 inhibits oral squamous cell carcinoma growth by targeting ZEB1.
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DOI：
10.12659/msm.893412
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发表时间：
2015-02-02
期刊：
影响因子：
--
通讯作者：
Qu L
中科院分区：
文献类型：
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作者：
Lei W;Liu YE;Zheng Y;Qu L
口腔鳞状细胞癌（OSCC）是全球第六大最常见的人类恶性肿瘤。为了开发新的治疗方法，需要阐明OSCC发病的潜在机制。miR-429在OSCC中的作用尚不清楚。采用qRT-PCR检测OSCC组织和细胞系中miR-429和ZEB1的表达水平。MiR-429通过miRNAs反义寡核苷酸（ASO）转染下调，miRNAs模拟物上调。MTT法检测细胞增殖情况。FACS分析显示细胞凋亡。目标基因通过生物信息学算法预测，并通过双荧光素酶报告基因试验确认。MiR-429在OSCC组织中下调，MiR-429过表达抑制OSCC细胞系生长，反之亦然。进一步，我们发现miR-429可以抑制锌指e -box结合同源盒1 （ZEB1）的表达，并且miR-429与ZEB1在OSCC组织中的表达呈负相关。我们的数据证明了miR-429在OSCC中的抑瘤作用，并可能提供一个潜在的治疗靶点，值得进一步研究。
Oral squamous cell carcinoma (OSCC) is the sixth most common human malignancy worldwide. To develop new therapeutics requires elucidation of the underlying mechanism of OSCC pathogenesis. The role of miR-429 in OSCC remains unknown. The level of miR-429 and ZEB1 in OSCC tissues and cell lines was measured by qRT-PCR. MiR-429 was down-regulated by miRNAs antisense oligonucleotides (ASO) transfection and up-regulated by miRNAs mimics. Cell proliferation was analyzed by MTT assay. Cell apoptosis was revealed by FACS analysis. Targeted genes were predicted by a bioinformatics algorithm and confirmed by a dual luciferase reporter assay. MiR-429 was down-regulated in OSCC tissues, and miR-429 overexpression inhibited OSCC cell lines growth and vice versa. Further, we found that miR-429 could inhibit zinc finger E-boxbinding homeobox 1 (ZEB1) expression, and that miR-429 and ZEB1 expression in OSCC tissues were negatively correlated. Our data demonstrate the tumor suppressor role of miR-429 in OSCC, and may provide a potential therapeutic target that warrants further investigation.