Antioxidant activity of extracts of Peh-Hue-Juwa-Chi-Cao in a cell free system
Antioxidant activity of extracts of Peh-Hue-Juwa-Chi-Cao in a cell free system
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DOI：
10.1142/s0192415x04001990
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发表时间：
2004-01-01
影响因子：
5.7
通讯作者：
Yang, JJ
中科院分区：
文献类型：
--
作者：
Lin, CC;Ng, LT;Yang, JJ
在传统中药制剂中，白花蛇舌草（HD）、伞房花耳草（HC）和五叶毛茛（MP）经常以“Peh-Hue-Juwa-Chi-Cao（PHJCC）”的名称互换使用。“虽然已经进行了研究来描述这些不同植物物种的治疗活性，但从未研究过它们的抗氧化活性。在这项研究中，我们的目的是评估这三种不同的植物材料的抗氧化和自由基清除活性。结果表明，在10 mg/ml浓度下，HD对FeCl_2-抗坏血酸诱导的大鼠肝匀浆脂质过氧化反应的抑制作用最强，其次是HC和MP。MP在1和3 mg/ml时表现出较弱的抗脂质过氧化活性。电子自旋共振（ESR）分析表明，其清除超氧阴离子的能力顺序为HC > HD > MP，但MP清除羟自由基的能力最强，其次为HC和HD。总之，在台湾用作PHJCC的所有三种植物都表现出不同的抗氧化和自由基清除活性;这些差异至少可以部分解释市场上PHJCC产品治疗特性的变化。
In traditional Chinese medicine preparations, Hedyotis diffusa (HD), Hedyotis corymbosa (HC) and Mollugo pentaphylla (MP) are often used interchangeably under the name of "Peh-Hue-Juwa-Chi-Cao (PHJCC)." Although studies have been conducted to characterize the therapeutic activities of these different plant species, their antioxidant activity has never been investigated. In this study, our aim was to evaluate the antioxidant and free radical scavenging activities of these three different plant materials. At a concentration of 10 mg/ml, results showed that HD possesses the strongest inhibition on the FeCl2-ascorbic acid induced lipid peroxidation in rat liver homogenate, followed by HC and MP. MP showed a weak anti-lipid peroxidation activity at 1 and 3 mg/ml. Using Electron Spin Resonance (ESR) analysis, the order of superoxide anion scavenging activity was HC > HD > MP However, MP was found to have the greatest hydroxyl radical scavenging activity compared to HC and HD. In conclusion, all three species used as PHJCC in Taiwan exhibited different antioxidant and free radical scavenging activities; these differences could explain, at least in part, the variation in therapeutic properties of PHJCC products in the market.