Leakage of macromolecules from guinea-pig tracheobronchial microcirculation. Effects of allergen, leukotrienes, tachykinins, and anti-asthma drugs.
Leakage of macromolecules from guinea-pig tracheobronchial microcirculation. Effects of allergen, leukotrienes, tachykinins, and anti-asthma drugs.
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豚鼠气管支气管微循环的大分子泄漏。
DOI：
10.1111/j.1748-1716.1986.tb07880.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
P. Andersson
中科院分区：
文献类型：
--
作者：
C. Persson;I. Erjefält;P. Andersson
麻醉豚鼠（正常或致敏与卵清蛋白产生IgE和IgG抗体）的气管支气管粘膜灌流（0.02 ml min-1，5 min）与盐水，介质，和（在致敏动物）卵清蛋白通过导管无创引入口。通过测量已在体内用99 Tcm标记的红细胞，并分析已静脉内给予的MW = 70，000的FITC-葡聚糖，定量血管内血池和切除的气管和邻近的主支气管中大分子的量。大分子的外渗被确定为分析的总含量减去计算的FITC-葡聚糖的血管内含量。辣椒素0.1 nmol外渗223 μ g FITC-葡聚糖/g气道组织湿重（P <0.001）。P物质0.1 nmol，41微克g-1（P大于0.05）; P物质0.3 nmol，142微克g-1（P小于0.001）;依地辛0.1 nmol，101微克g-1卵清蛋白0.1 μ g、179 μ g g g-1 LTC4 0.2 pmol，180 μ g g g-1（P <0.001）; LTD4 0.2 pmol，223 μ g ml-1（P <0.001）。支气管和气管同样受到这些制剂的影响。预先灌注（0.02 ml min-1，30 min）特布他林0.06 nmol，恩丙茶碱12 nmol，或利多卡因6 nmol显着降低辣椒素的效果。恩普罗茶碱也显著降低了LTC 4的作用。本研究中的外渗程度小于通过组织湿干重量比变化检测到的外渗程度。目前的数据支持这样的观点，即气管支气管血管对大分子的渗透性受到生理和药理学的控制。
The tracheobronchial mucosa of anaesthetized guinea-pigs (normal or sensitized with ovalbumin to produce IgE and IgG antibodies) was superfused (0.02 ml min-1, 5 min) with saline, mediators, and (in sensitized animals) ovalbumin via a catheter atraumatically introduced orally. The intravascular blood pool and amount of macromolecules in excised trachea and adjoining main bronchi were quantified by measuring erythrocytes, that had been labelled in vivo with 99Tcm, and analysing for FITC-dextran, MW = 70,000, that had been given i.v. Extravasation of macromolecules was determined as the analysed total content minus the calculated intravascular content of FITC-dextran. Capsaicin 0.1 nmol extravasated 223 micrograms of FITC-dextran per g wet weight of airway tissue (P less than 0.001). Substance P 0.1 nmol, 41 micrograms g-1 (P greater than 0.05); substance P 0.3 nmol, 142 micrograms g-1 (P less than 0.001); eledoisine 0.1 nmol, 101 micrograms g-1 (P less than 0.01); ovalbumin 0.1 microgram, 179 micrograms g-1 (P less than 0.001); LTC4 0.2 pmol, 180 micrograms g-1 (P less than 0.001); LTD4 0.2 pmol 223 micrograms ml-1 (P less than 0.001). Bronchi and trachea were similarly affected by these agents. Prior superfusion (0.02 ml min-1, 30 min) with terbutaline 0.06 nmol, enprofylline 12 nmol, or lidocaine 6 nmol significantly reduced the effect of capsaicin. Enprofylline also reduced significantly the effect of LTC4. The degree of extravasation in this study was smaller than could be detected by changes in tissue wet to dry weight ratios. The present data support the view that tracheobronchial vascular permeability to macromolecules is subject to physiological and pharmacological control.