Protein synthesis in isolated enterocytes from septic or endotoxaemic rats: regulation by glutamine.
Protein synthesis in isolated enterocytes from septic or endotoxaemic rats: regulation by glutamine.
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脓毒症或内毒素血症大鼠分离的肠细胞中的蛋白质合成：谷氨酰胺的调节。
DOI：
10.1042/cs0890311
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hasselgren,PO
中科院分区：
文献类型：
--
作者：
Higashiguchi,T;Noguchi,Y;Meyer,T;Fischer,JE;Hasselgren,PO
1. 我们研究了脓毒症对大鼠小肠分离肠细胞的影响及谷氨酰胺对蛋白质合成的调节作用。2. 盲肠结扎穿刺致脓毒症；对照组采用假手术。从空肠中分离肠细胞，在含有[3H]苯丙氨酸的培养基中培养。3. 盲肠结扎和穿刺16小时后，肠细胞中绒毛尖端和中部以及隐窝的蛋白质合成分别增加了65%，89%和137%，以放射性掺入蛋白质来测量。4. 在孵育的肠细胞中添加谷氨酰胺以剂量依赖的方式刺激蛋白质合成，这种作用在脓毒症大鼠的隐窝细胞中最为明显。谷氨酰胺对蛋白质合成的影响被等摩尔浓度的乙酰乙酸或3-羟基丁酸复制，这两种物质都可以作为肠细胞的燃料，并被谷氨酰胺酶抑制剂6-重氮-5-氧- l -去甲亮氨酸阻断。5. 结果表明，脓毒症刺激肠细胞中的蛋白质合成，而谷氨酰胺可能通过提供能量来调节相同细胞中的蛋白质合成。
1. We studied the effect of sepsis and the regulation by glutamine of protein synthesis in enterocytes isolated from the small intestine of rats. 2. Sepsis was induced by caecal ligation and puncture; control rats were sham operated. Enterocytes were isolated from the jejunum and incubated in a medium containing [3H] phenylalanine. 3. Sixteen hours after caecal ligation and puncture, protein synthesis, measured as incorporation of radioactivity into protein, was increased by 65%, 89% and 137% respectively in enterocytes from the tips and mid-portions of the villi and from the crypts. 4. Addition of glutamine to incubated enterocytes stimulated protein synthesis in a dose-dependent manner, and this effect was most pronounced in crypt cells from septic rats. The effect of glutamine on protein synthesis was duplicated by equimolar concentrations of acetoacetate or 3-hydroxybutyrate, both of which may serve as fuel for enterocytes, and was blocked by the glutaminase inhibitor 6-diazo-5-oxo-L-norleucine. 5. The results suggest that sepsis stimulates protein synthesis in enterocytes and that glutamine regulates protein synthesis in the same cells, probably by energy provision.