Trypanosoma rangeli isolates of bats from Central Brazil: Genotyping and phylogenetic analysis enable description of a new lineage using spliced-leader gene sequences
Trypanosoma rangeli isolates of bats from Central Brazil: Genotyping and phylogenetic analysis enable description of a new lineage using spliced-leader gene sequences
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DOI：
10.1016/j.actatropica.2008.11.005
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发表时间：
2009-03-01
期刊：
影响因子：
2.7
通讯作者：
Teixeira, M. M. G.
中科院分区：
文献类型：
--
作者：
da Silva, F. Maia;Marcili, A.;Teixeira, M. M. G.
兰氏锥虫感染了许多哺乳动物目，但在通常被许多锥虫种寄生的翼手目中从未有过可靠的描述。在这里，我们描述了从巴西中部捕获的蝙蝠身上发现的锥虫，鉴定为T rangeli，.T。dionisii， T cruzimarinkellei和T cruzi。从Platyrrhinus lineatus分离的Tra643和从Artibeus plamirostris分离的Tra1719经形态学、生物学和分子分析鉴定为T rangeli，并进行了系统发育分析。利用SSU rDNA序列分析将这些蝙蝠锥虫与其他宿主的T rangeli聚集在一起，并将它们与蝙蝠的其他锥虫分离开来。基于pcr扩增的基因间剪接前导基因序列的长度和序列多态性的基因分型将Tra1719分配给A谱系，而Tra643被证明是一个新的基因型，并被分配给e谱系。据我们所知，这两个分离株是最早来自蝙蝠的T rangeli，也是第一个来自巴西中部的分子特征分离株。本研究捕获的大菱鲆被发现感染了朗格里T和克氏T。(c) 2008 Elsevier B.V.版权所有
Trypanosoma rangeli infects several mammalian orders but has never confidently been described in Chiroptera, which are commonly parasitized by many trypanosome species. Here, we described trypanosomes from bats captured in Central Brazil identified as T rangeli,.T. dionisii, T cruzimarinkellei and T cruzi. Two isolates, Tra643 from Platyrrhinus lineatus and Tra1719 from Artibeus plamirostris were identified as T rangeli by morphological, biological and molecular methods, and confirmed by phylogenetic analyses. Analysis using SSU rDNA sequences clustered these bat trypanosomes together with T rangeli from other hosts, and separated them from other trypanosomes from bats. Genotyping based on length and sequence polymorphism of PCR-amplified intergenic spliced-leader gene sequences assigned Tra1719 to the lineage A whereas Tra643 was shown to be a new genotype and was assigned to the new lineage E. To our knowledge, these two isolates are the earliest T rangeli from bats and the first isolates from Central Brazil molecularly characterized. Rhodnius stali captured for this study was found infected by T rangeli and T cruzi. (c) 2008 Elsevier B.V. All rights reserved.