Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits
Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits
复制标题
DOI：
10.1128/aem.01052-10
复制
发表时间：
2010-10-01
影响因子：
4.4
通讯作者：
Leon-Quintero, Monica C.
中科院分区：
文献类型：
--
作者：
Hernandez-Sanabria, Emma;Guan, Le Luo;Leon-Quintero, Monica C.
瘤胃微生物结构和功能对宿主生理学的影响仍然知之甚少。本研究旨在通过使用独立于培养的方法将细菌多样性与发酵测量和饲料效率性状（包括干物质摄入量、饲料转化率、平均日增重和剩余饲料摄入量）相关联，来研究瘤胃微生物区系与宿主之间的相互作用。从 58 头低能量饮食下饲养的公牛身上收集的瘤胃液中扩增通用细菌部分 16S rRNA 基因产物，并进行 PCR 变性梯度凝胶电泳 (DGGE) 分析。使用多变量统计分析将特定 PCR-DGGE 条带与各种饲料效率性状和代谢物联系起来。挥发性脂肪酸谱分析表明，丁酸与每日干物质摄入量呈正相关（P < 0.05），并且在低效率动物中浓度往往较高（P = 0.10），而异戊酸与剩余采食量相关（P < 0.05）。我们的结果表明，特定的细菌及其在瘤胃中的代谢可能会导致低能量饮食下宿主饲料效率的差异。这是第一项将代表特定细菌的 PCR-DGGE 条带与牛瘤胃中的代谢物以及宿主饲料效率特征相关联的研究。
The influence of rumen microbial structure and functions on host physiology remains poorly understood. This study aimed to investigate the interaction between the ruminal microflora and the host by correlating bacterial diversity with fermentation measurements and feed efficiency traits, including dry matter intake, feed conversion ratio, average daily gain, and residual feed intake, using culture-independent methods. Universal bacterial partial 16S rRNA gene products were amplified from ruminal fluid collected from 58 steers raised under a low-energy diet and were subjected to PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Multivariate statistical analysis was used to relate specific PCR-DGGE bands to various feed efficiency traits and metabolites. Analysis of volatile fatty acid profiles showed that butyrate was positively correlated with daily dry matter intake (P < 0.05) and tended to have higher concentration in inefficient animals (P = 0.10), while isovalerate was associated with residual feed intake (P < 0.05). Our results suggest that particular bacteria and their metabolism in the rumen may contribute to differences in host feed efficiency under a low-energy diet. This is the first study correlating PCR-DGGE bands representing specific bacteria to metabolites in the bovine rumen and to host feed efficiency traits.