The transcriptional profile of early to middle sporulation in Bacillus subtilis
The transcriptional profile of early to middle sporulation in Bacillus subtilis
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DOI：
10.1073/pnas.140209597
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发表时间：
2000-07-05
影响因子：
11.1
通讯作者：
Youngman, P
中科院分区：
文献类型：
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作者：
Fawcett, P;Eichenberger, P;Youngman, P
枯草芽孢杆菌的孢子形成受基因转录的整体变化控制。我们使用代表枯草芽孢杆菌染色体中大约 96% 的预测开放阅读框的尼龙基质 DNA 阵列，将野生型细胞的转录物模式与孢子形成转录因子 Spo0A 或 sigma(F) 突变细胞的转录物模式进行比较。我们发现 520 个基因的转录水平至少 3 倍依赖于 Spo0A，但不依赖于 sigma(F)，另外 66 个基因的转录水平依赖于两种调节蛋白。使用两种策略来帮助指定基因来直接控制特定的发育调节蛋白。在一种方法中，我们分析了在生长过程中人工产生组成型活性形式的 Spo0A 对全局基因表达的影响。在第二种方法中，使用隐马尔可夫模型来识别可能被 Spo0A、sigma(F) 或第三个孢子形成转录因子 sigma(E) 激活的启动子。除了检测已知的孢子形成基因之外，我们还鉴定了许多功能未知的基因，其表达和调控模式表明它们可能参与孢子形成。破坏两个新发现的基因，yabP和yabQ，在后期阻止孢子形成。
Spore formation by Bacillus subtilis is governed by global changes in gene transcription. We used nylon-substrate DNA arrays representing approximate to 96% of the predicted open reading frames in the B. subtilis chromosome to compare the pattern of transcripts from wild-type cells with the pattern from cells mutant for the sporulation transcription factors Spo0A or sigma(F). We found 520 genes whose transcript levels were at least 3-fold dependent on Spo0A but not on sigma(F), and an additional 66 genes whose transcript levels were dependent upon both regulatory proteins. Two strategies were used to help assign genes to the direct control of a particular developmental regulatory protein. In one approach, we analyzed the effects on global gene expression of artificially producing a constitutively active form of Spo0A during growth. In a second approach, Hidden Markov models were used to identify promoters likely to be activated by Spo0A, sigma(F), or a third sporulation transcription factor, sigma(E). In addition to detecting known sporulation genes, we identified many genes of unknown function whose patterns of expression and regulation suggest that they could be involved in sporulation, Disruption of two such newly identified genes, yabP and yabQ, blocked sporulation at a late stage.