Monensin and Nisin Affect Rumen Fermentation and Microbiota Differently In Vitro.
Monensin and Nisin Affect Rumen Fermentation and Microbiota Differently In Vitro.
复制标题
莫能菌素和乳链菌肽在体外对瘤胃发酵和微生物群的影响不同
DOI：
10.3389/fmicb.2017.01111
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发表时间：
2017
影响因子：
5.2
通讯作者：
Zhu W
中科院分区：
文献类型：
--
作者：
Shen J;Liu Z;Yu Z;Zhu W
Nisin, a bacteriocin, is a potential alternative to antibiotics to modulate rumen fermentation. However, little is known about its impacts on rumen microbes. This study evaluated the effects of nisin (1 and 5 μM) on in vitro rumen fermentation characteristics, microbiota, and select groups of rumen microbes in comparison with monensin (5 μM), one of the most commonly used ionophores in ruminants. Nisin had greater effects than monensin in inhibiting methane production and decreasing acetate/propionate ratio. Unlike monensin, nisin had no adverse effect on dry matter digestibility. Real-time PCR analysis showed that both monensin and nisin reduced the populations of total bacteria, fungi, and methanogens, while the population of protozoa was reduced only by monensin. Principal component analysis of bacterial 16S rRNA gene amplicons showed a clear separation between the microbiota shaped by monensin and by nisin. Comparative analysis also revealed a significant difference in relative abundance of some bacteria in different taxa between monensin and nisin. The different effects of monensin and nisin on microbial populations and bacterial communities are probably responsible for the discrepancy in their effects on rumen fermentation. Nisin may have advantages over monensin in modulating ruminal microbial ecology and reducing ruminant methane production without adversely affecting feed digestion, and thus it may be used as a potential alternative to monensin fed to ruminants.
