Autonomous dynamic control of DNA nanostructure self-assembly
Autonomous dynamic control of DNA nanostructure self-assembly
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DOI：
10.1038/s41557-019-0251-8
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发表时间：
2019-06-01
期刊：
影响因子：
21.8
通讯作者：
Franco, Elisa
中科院分区：
文献类型：
--
作者：
Green, Leopold N.;Subramanian, Hari K. K.;Franco, Elisa
生物细胞通常使用动态信号和调节网络来重新配置其形状，这些信号和调节网络通过感知、处理和传输来自环境的信息的分子组件来指导时间和空间上的自组装过程。类似的策略可用于在合成材料中实现逼真的行为。核酸纳米技术通过各种传感器、逻辑和动态组件以及自组装结构为实现这一目标提供了一条有前途的途径。在这里，通过利用动态和结构 DNA 纳米技术，我们展示了 DNA 纳米管自组装的动态控制——DNA 纳米管是一种众所周知的可编程 DNA 纳米结构。纳米管的组装和拆卸由最小的合成基因系统控制，包括自主分子振荡器。我们使用粗粒度计算模型来捕获响应核酸电路输入的纳米管长度分布动态。我们希望这些结果可用于响应性核酸材料的开发，并在生物材料科学、纳米制造和药物输送方面具有潜在的应用。
Biological cells routinely reconfigure their shape using dynamic signalling and regulatory networks that direct self-assembly processes in time and space, through molecular components that sense, process and transmit information from the environment. A similar strategy could be used to enable life-like behaviours in synthetic materials. Nucleic acid nanotechnology offers a promising route towards this goal through a variety of sensors, logic and dynamic components and self-assembling structures. Here, by harnessing both dynamic and structural DNA nanotechnology, we demonstrate dynamic control of the self-assembly of DNA nanotubes-a well-known class of programmable DNA nanostructures. Nanotube assembly and disassembly is controlled with minimal synthetic gene systems, including an autonomous molecular oscillator. We use a coarse-grained computational model to capture nanotube length distribution dynamics in response to inputs from nucleic acid circuits. We hope that these results may find use for the development of responsive nucleic acid materials, with potential applications in biomaterials science, nanofabrication and drug delivery.