INHIBITION OF PHAGOCYTOTIC METABOLIC CHANGES OF LEUKOCYTES BY AN INTRACELLULAR CALCIUM-ANTAGONIST 8-(N,N-DIETHYLAMINO)-OCTYL-3,4,5-TRIMETHOXYBENZOATE
INHIBITION OF PHAGOCYTOTIC METABOLIC CHANGES OF LEUKOCYTES BY AN INTRACELLULAR CALCIUM-ANTAGONIST 8-(N,N-DIETHYLAMINO)-OCTYL-3,4,5-TRIMETHOXYBENZOATE
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DOI：
10.1016/0006-291x(79)91503-1
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
MINAKAMI, S
中科院分区：
文献类型：
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作者：
MATSUMOTO, T;TAKESHIGE, K;MINAKAMI, S
用细胞内钙拮抗剂8-（N，N-二乙基氨基）-辛基-3,4,5-三甲氧基苯甲酸酯研究了钙在调节[豚鼠腹膜]白细胞生成和释放超氧化物活性中的作用。该拮抗剂抑制钙离子载体A23187诱导的超氧阴离子的释放，并通过钙离子的加入缓解这种抑制作用。细胞松弛素D或细菌摄入诱导的释放同样受到钙拮抗剂的抑制。细胞内钙易位调节白细胞吞噬代谢活性的假说得到支持。离子载体诱导的颗粒酶释放也受到钙拮抗剂的抑制。
The role of Ca in regulating the activity of [guinea pig peritoneal] leukocytes to generate and release superoxide was studied by using an intracellular Ca-antagonist, 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate. The antagonist inhibited the release of superoxide anions induced by a Ca-ionophore A23187 and the inhibition was relieved by the addition of Ca ions. The release induced by cytochalasin D or by the ingestion of bacteria was similarly inhibited by the Ca-antagonist. The hypothesis that an intracellular translocation of Ca is regulating the phagocytotic metabolic activity of leukocytes was supported. The release of granule enzymes induced by the ionophore was also inhibited by the Ca-antagonist.