Protein-Templated Peptide Ligation**
Protein-Templated Peptide Ligation**
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DOI：
10.1002/anie.201400681
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发表时间：
2014-04-22
影响因子：
16.6
通讯作者：
Grossmann, Tom N.
中科院分区：
文献类型：
--
作者：
Brauckhoff, Nicolas;Hahne, Gernot;Grossmann, Tom N.
分子模板结合了特定的反应物，从而增加了它们的有效浓度并加速了相应的反应。这一概念已经成功地应用于一些化学问题，特别是核酸模板反应。我们提出了第一个蛋白质模板化反应，允许两个肽的N-末端连接。在蛋白质模板的存在下，连接反应加速了三个数量级以上。模板化反应具有很高的选择性，并在粗细胞裂解液中进行的蛋白质标记反应中证明了其稳健性。
Molecular templates bind particular reactants, thereby increasing their effective concentrations and accelerating the corresponding reaction. This concept has been successfully applied to a number of chemical problems with a strong focus on nucleic acid templated reactions. We present the first protein-templated reaction that allows N-terminal linkage of two peptides. In the presence of a protein template, ligation reactions were accelerated by more than three orders of magnitude. The templated reaction is highly selective and proved its robustness in a protein-labeling reaction that was performed in crude cell lysate.