INDUCTION OF POST-AGGREGATIVE DIFFERENTIATION IN DICTYOSTELIUM DISCOIDEUM BY CAMP - EVIDENCE OF INVOLVEMENT OF THE CELL-SURFACE CAMP RECEPTOR
INDUCTION OF POST-AGGREGATIVE DIFFERENTIATION IN DICTYOSTELIUM DISCOIDEUM BY CAMP - EVIDENCE OF INVOLVEMENT OF THE CELL-SURFACE CAMP RECEPTOR
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DOI：
10.1016/s0014-4827(85)80012-4
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发表时间：
1985-01-01
影响因子：
3.7
通讯作者：
VANDRIEL, R
中科院分区：
文献类型：
--
作者：
SCHAAP, P;VANDRIEL, R
已知外源 cAMP 在正常发育受阻的条件下诱导盘基网柄菌的聚集后分化。我们分析了环核苷酸特异性、cAMP信号调节的效果以及诱导聚集后分化的两个独立标记物（即通过单克隆抗体检测到的前孢子细胞特异性抗原）和糖原磷酸化酶的活性的剂量反应关系。我们的结果证实，诱导这些标记物需要高浓度的 cAMP (10-6-10-3M)。这些细胞不适应 cAMP 信号。诱导的环核苷酸特异性与细胞表面cAMP受体的特异性一致，但与细胞内cAMP依赖性蛋白激酶的特异性非常不同。因此，cAMP 不太可能渗入细胞并直接激活 cAMP 依赖性蛋白激酶。相反，cAMP 对聚集后分化的诱导似乎是由细胞表面 cAMP 受体介导的。
Exogenous cAMP is known to induce post-aggregative differentiation in Dictyostelium discoideum under conditions that normal development is blocked. We have analyzed the cyclic nucleotide specificity, the effect of modulation of the cAMP signal and the dose-response relationship of the induction of two independent markers of post-aggregative differentiation, i.e., a prespore cell-specific antigen detected by a monoclonal antibody, and the activity of glycogen phosphorylase. Our results confirm that high concentrations of cAMP (10-6-10-3M) are required for the induction of these markers. The cells are shown not to adapt to the cAMP signal. The cyclic nucleotide specificity of induction agrees with the specificity of the cell surface cAMP receptor, but is very dissimilar to the specificity of the intracellular cAMP-dependent protein kinase. It is thus unlikely that cAMP leaks into the cell and activates the cAMP-dependent protein kinase directly. Instead, the induction of post-aggregative differentiation by cAMP seems to be mediated by cell surface cAMP receptors.