Endogenous amino acid pool sizes in mouse eggs and preimplantation embryos.
Endogenous amino acid pool sizes in mouse eggs and preimplantation embryos.
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DOI：
10.1530/jrf.0.0610387
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发表时间：
1981-03
期刊：
影响因子：
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通讯作者：
G. Schultz;P. Kaye;D. McKay;M. Johnson
中科院分区：
文献类型：
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作者：
G. Schultz;P. Kaye;D. McKay;M. Johnson
通过邻苯二甲醛与伯胺反应的荧光产物的估计，直接测量了小鼠卵、8-细胞胚胎和囊胚样品中17种氨基酸的内源性池大小。检测到高水平的牛磺酸、甘氨酸、丙氨酸、谷氨酸和天冬氨酸。在向胚泡的过渡期间，大多数氨基酸池增加2-3倍，但牛磺酸和甘氨酸池分别下降至卵值的约50%和10%。卵丘中氨基酸的分布与卵和胚胎中的氨基酸分布相似，而与血清中的氨基酸分布不同。
The endogenous pool sizes of 17 amino acids were measured directly in samples of mouse eggs, 8-cell embryos and blastocysts by estimation of the fluorescent product of the reaction of o-phthalaldehyde and primary amines. Taurine, glycine, alanine, glutamate and aspartate were detected at high levels. During the transition to the blastocyst, most amino acid pools increased 2-3-fold, but the taurine and glycine pools decreased to about 50 and 10%, respectively, of the egg value. The amino acid distribution in cumulus masses was similar to that of the egg and embryo samples but different from that of serum.