IMMUNOFLUORESCENT IDENTIFICATION OF FIBRONECTIN AND FIBRINOGEN FIBRIN IN EXPERIMENTAL MYOCARDIAL-INFARCTION
IMMUNOFLUORESCENT IDENTIFICATION OF FIBRONECTIN AND FIBRINOGEN FIBRIN IN EXPERIMENTAL MYOCARDIAL-INFARCTION
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DOI：
10.1016/0022-2828(90)90955-2
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发表时间：
1990-05-01
影响因子：
5
通讯作者：
KOTELIANSKY, VE
中科院分区：
文献类型：
--
作者：
SHEKHONIN, BV;GURIEV, SB;KOTELIANSKY, VE
本文应用免疫荧光技术研究了纤维蛋白原/纤维蛋白和纤维连接蛋白在实验性心肌梗塞部位的分布。与正常心肌不同，冠状动脉结扎后3和12至24小时，检测到纤维蛋白原/纤维蛋白和血浆纤维连接蛋白浸润坏死的心肌细胞。纤维蛋白原/纤维蛋白和血浆纤连蛋白构成肉芽组织的“初级基质”。在实验性梗塞后第三天，开始合成细胞纤连蛋白。其在肉芽组织的细胞外基质（ECM）中的含量在第7天至第15天显著增加。梗死后30天，瘢痕组织细胞外基质中纤维连接蛋白的含量显著降低。纤维蛋白原/纤维蛋白在心肌梗死区的肉芽组织中不断被识别。而在瘢痕组织的细胞外基质中，其含量逐渐减少。
Distribution of fibrinogen/fibrin and fibronectin in regions of experimental myocardial infarction were studied by the immunofluorescence technique. Distinct from normal myocardium 3 and 12 to 24 h after coronary artery ligation infiltration of necrotized cardiomyocytes by fibrinogen/fibrin and plasma fibronectin was detected. Fibrinogen/fibrin and plasma fibronectin constitute "primary matrix" of granulation tissue. On the third day after experimental infarction, synthesis of cellular fibronectin begins. Its content in the extracellular matrix (ECM) of granulation tissue significantly increases on days 7 to 15. The amount of fibronectin in the ECM of developing scar tissue dramatically decreases 30 days after infarction. Fibrinogen/fibrin was continually identified in granulation tissue in zones of myocardial infarction. However, its amount in the ECM of developing scar tissue gradually decreased.