LOCALIZATION OF DNASE-I HYPERSENSITIVE SITES IN THE HUMAN ESTROGEN-RECEPTOR GENE CORRELATES WITH THE TRANSCRIPTIONAL ACTIVITY OF 2 DIFFERENTIALLY USED PROMOTERS
LOCALIZATION OF DNASE-I HYPERSENSITIVE SITES IN THE HUMAN ESTROGEN-RECEPTOR GENE CORRELATES WITH THE TRANSCRIPTIONAL ACTIVITY OF 2 DIFFERENTIALLY USED PROMOTERS
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DOI：
10.1677/jme.0.0100269
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发表时间：
1993-06-01
影响因子：
3.5
通讯作者：
GUSTAFSSON, JA
中科院分区：
文献类型：
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作者：
GRANDIEN, KFH;BERKENSTAM, A;GUSTAFSSON, JA
人类雌激素受体（hER）基因最近已被证明是从两个不同的启动子转录。为了研究hER基因启动子区，我们首先分离并测序了hER基因5 '区的3.3kb。DNA酶I超敏反应实验与细胞核的ER表达细胞系MCF-7映射三个不同的超敏位点，位于附近的两个人ER启动子先前确定。然而，在另一ER阳性细胞系ZR-75 - 1中，仅在下游启动子区域观察到DNA酶I超敏反应，表明两种细胞系在启动子使用方面存在差异。聚合酶链反应介导的检测从两个不同的启动子转录的mRNA表现出转录从下游启动子在两个细胞系研究。然而，从上游启动子转录的mRNA只能在MCF-7细胞中检测到。因此，DNA酶I超敏反应的模式与hER基因中两个启动子的转录活性相关。基于这些数据，我们认为，差异启动子的使用可能是一个新的水平的调节hER基因。
The human oestrogen receptor (hER) gene has recently been shown to be transcribed from two different promoters. In order to study the hER gene promoter region we initially isolated and sequenced 3.3 kb of the 5' region of the hER gene. DNase I hypersensitivity experiments with nuclei from the ER-expressing cell line MCF-7 mapped three different hypersensitive sites, located in the vicinity of the two hER promoters previously identified. However, in another ER-positive cell line, ZR-75-1, DNase I hypersensitivity was observed only in the region of the downstream promoter, suggesting differences between the two cell lines in promoter usage. Polymerase chain reaction-mediated detection of mRNAs transcribed from the two different promoters demonstrated transcripts from the downstream promoter in both the cell lines studied. However, mRNAs transcribed from the upstream promoter could only be detected in MCF-7 cells. Thus, the pattern of DNase I hypersensitivity correlates with the transcriptional activity of the two promoters in the hER gene. Based on these data, we suggest that differential promoter usage might be a novel level of regulation of the hER gene.