Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization
Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization
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免疫亲和柱净化和柱前衍生化后气相色谱-电子捕获法检测中草药及相关产品中 T-2 和 HT-2 毒素的验证
DOI：
10.1016/j.foodchem.2011.10.073
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发表时间：
2012-05-01
期刊：
影响因子：
8.8
通讯作者：
Yang, Meihua
中科院分区：
文献类型：
--
作者：
Kong, Weijun;Zhang, Xiaofei;Yang, Meihua
建立了一种灵敏、重现性好且准确的气相色谱 - 电子捕获检测（GC - ECD）方法，用于在免疫亲和柱（IAC）净化以及用N - 七氟丁酰基咪唑（HFBI）进行柱前衍生化后，同时测定中药材（CHMs）及相关产品中的T - 2和HT - 2毒素。然后，应用气相色谱 - 质谱联用仪（GC - MS）对阳性结果和干扰峰进行确认。T - 2和HT - 2毒素的检测限（LODs）分别为1.88纳克/克和0.47纳克/克，不同中药材的回收率在89.2%至99.1%之间，相对标准偏差（RSD）……
A sensitive, reproducible and accurate gas chromatography-electron capture detection (GC-ECD) method was developed for simultaneous determination of T-2 and HT-2 toxins in Chinese herbal medicines (CHMs) and related products after immunoaffinity column (IAC) clean-up and pre-column derivatization with N-heptafluoro-butyryl imidazole (HFBI). Then, gas chromatography-spectrometry spectrometer (GC-MS) was applied to confirm the positive results and interfering peaks. The limits of detection (LODs) for T-2 and HT-2 toxins were 1.88 and 0.47 ng/g, and the recoveries for different CHMs ranged from 89.2% to 99.1% with relative standard deviation (RSD)