Transcriptome and proteome analyses reveal the regulatory networks and metabolite biosynthesis pathways during the development of Tolypocladium guangdongense
Transcriptome and proteome analyses reveal the regulatory networks and metabolite biosynthesis pathways during the development of Tolypocladium guangdongense
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DOI：
10.1016/j.csbj.2020.07.014
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发表时间：
2020-07
影响因子：
6
通讯作者：
Gangzheng Wang;Min Li;Chenghua Zhang;Huijiao Cheng;Yu Gao;W. Deng;Taihui Li
中科院分区：
文献类型：
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作者：
Gangzheng Wang;Min Li;Chenghua Zhang;Huijiao Cheng;Yu Gao;W. Deng;Taihui Li
广东弯颈霉（Tolypocladium guangdongenensis）是一种营养价值和药用价值都很高的中药真菌，其代谢产物与中华蛇蜕菌（Ophiocoracidum sinensis）相似。虽然T. guangdongense的报道，但对子实体发育的调控网络和代谢产物的生物合成途径知之甚少。为了解决这一问题，本研究对T.进行广东根管治疗。总共有9076个基因被发现表达，2040个蛋白被鉴定。在菌丝期和各期之间存在大量差异表达的基因。有趣的是，转录组学和蛋白质组学数据之间的相关性很低，这表明转录后过程在T.广东话。在发育过程中差异表达的基因/蛋白质中，有许多热休克蛋白和转录因子。此外，有许多蛋白质参与萜类，麦角固醇，腺苷和多糖的生物合成，也表现出显着的下调，其表达水平在发育过程中。此外，色氨酸和色胺都存在于较高的水平在原基阶段。然而，吲哚-3-乙酸（IAA）的水平不断下降，随着发展的进行，和参与IAA生物合成的酶也明显差异下调。这些数据对研究大型真菌发育的分子机制以及大型真菌的工业和医药应用具有重要意义。
Tolypocladium guangdongensehas a similar metabolite profile toOphiocordyceps sinensis, a highly regarded fungus used for traditional Chinese medicine with high nutritional and medicinal value. Although the genome sequence ofT. guangdongensehas been reported, relatively little is known about the regulatory networks for fruiting body development and about the metabolite biosynthesis pathways. In order to address this, an analysis of transcriptome and proteome at differential developmental stages ofT. guangdongensewas performed. In total, 9076 genes were found to be expressed and 2040 proteins were identified. There were a large number of genes that were significantly differentially expressed between the mycelial stage and the stages. Interestingly, the correlation between the transcriptomic and proteomic data was low, suggesting the importance of the post-transcriptional processes in the growth and development ofT. guangdongense. Among the genes/proteins that were both differentially expressed during the developmental process, there were numerous heat shock proteins and transcription factors. In addition, there were numerous proteins involved in terpenoid, ergosterol, adenosine and polysaccharide biosynthesis that also showed significant downregulation in their expression levels during the developmental process. Furthermore, both tryptophan and tryptamine were present at higher levels in the primordium stage. However, indole-3-acetic acid (IAA) levels continuously decreased as development proceeded, and the enzymes involved in IAA biosynthesis were also clearly differentially downregulated. These data could be meaningful in studying the molecular mechanisms of fungal development, and for the industrial and medicinal application of macro-fungi.