The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells.
The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells.
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DOI：
10.2478/s11658-012-0031-6
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发表时间：
2012-12
影响因子：
8.3
通讯作者：
Autexier C
中科院分区：
文献类型：
--
作者：
Trapp-Fragnet L;Marie-Egyptienne DT;Fakhoury J;Rasschaert D;Autexier C
脊椎动物最小的端粒酶由蛋白质组分(端粒酶逆转录酶，TERT)和RNA组分(端粒酶RNA，TR)组成。这两个亚基的表达足以在体外重建端粒酶活性，而由端粒酶相关蛋白组成的全酶的形成是维持适当端粒长度所必需的。以往的报道证实了人类端粒酶复合体的高度加工性和人类端粒酶逆转录酶(HTERT)的种间相容性。在这项研究中，我们测试了唯一已知的与人端粒酶相关的病毒端粒酶RNA亚单位(VTR)在无细胞系统和人类细胞中的功能。当VTR与hTERT在无细胞环境中组装时，它能够与hTERT相互作用并重建端粒酶活性。然而，在人类细胞中，VTR不能重建端粒酶活性，也不能在人端粒酶复合体中检测到，这表明VTR不能与组成人端粒酶全酶的蛋白质正确地相互作用。
The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.
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影响因子：
14.9
作者：
Huard, S;Moriarty, TJ;Autexier, C
通讯作者：
Autexier, C
影响因子：
9.2
作者：
Beattie, TL;Zhou, W;Harrington, L
通讯作者：
Harrington, L
影响因子：
6.7
作者：
Kaufer, Benedikt B.;Arndt, Sina;Jarosinski, Keith W.
通讯作者：
Jarosinski, Keith W.
影响因子：
11.4
作者：
Autexier, C;Pruzan, R;Greider, CW
通讯作者：
Greider, CW
影响因子：
16
作者：
Fu, D;Collins, K
通讯作者：
Collins, K