Heterologous expression of chrysanthemum TOPLESS corepressor CmTPL1-1 alters meristem maintenance and organ development in Arabidopsis thaliana
Heterologous expression of chrysanthemum TOPLESS corepressor CmTPL1-1 alters meristem maintenance and organ development in Arabidopsis thaliana
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DOI：
10.1016/j.plaphy.2020.10.031
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发表时间：
2020-12-01
影响因子：
6.5
通讯作者：
Chen, Fadi
中科院分区：
文献类型：
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作者：
Chong, Xinran;Guan, Yunxiao;Chen, Fadi
toppless (TPL)/ toppless相关（TPR）共抑制因子是植物生长发育的重要调控因子，但其在菊花（chrysanthemum morifolium）中的功能尚不清楚。本研究对菊花TPL/TPR家族基因CmTPL1-1进行了鉴定。该基因编码一个1135个氨基酸的多肽，包含一个保守的n端结构域和两个c端WD40结构域。CmTPL1-1在酵母中没有转录活性，定位实验表明它定位在洋葱表皮细胞的细胞核中。转录谱分析表明，该基因在茎尖表达量最高。CmTPL1-1在拟南芥中异种表达产生叶片变小、角质部缩短、分生组织数量增加、花瓣分布不对称、雄蕊数量减少等多效表型。在转基因植物中，4个AtARFs在转录水平上下调，6个AtIAAs和2个AtGH3s在转录水平上上调；此外，三个关键的I类KNOX同源盒（KNOX）基因的表达上调。此外，通过酵母双杂交筛选菊花cDNA文库，我们发现CmTPL1-1可以与CmWOX4、CmLBD38和CmLBD36相互作用，并通过双分子荧光互补（bic）实验证实了这些相互作用。综上所述，我们推测CmTPL1-1的异源表达通过与植物生长素信号相互作用调控拟南芥的生长发育。
TOPLESS (TPL)/TOPLESS-related (TPR) corepressors are important regulators of plant growth and development, but their functions in chrysanthemum (Chrysanthemum morifolium) are currently unclear. In this study, a chrysanthemum TPL/TPR family gene, designated CmTPL1-1, was characterized. This gene encodes an 1135-amino-acid polypeptide harboring a conserved N-terminal domain and two C-terminal WD40 domains. CmTPL1-1 showed no transcriptional activity in yeast, and a localization experiment indicated that it localized to the nuclei in onion epidermal cells. Transcript profiling established that the gene was most highly expressed in the stem apex. The hetemlogous expression of CmTPL1-1 in Arabidopsis thaliana produced a pleiotropic phenotype, including smaller leaves, shorter siliques, increased meristem number, asymmetrical petal distribution and reduced stamen number. In transgenic plants, four AtARFs were downregulated, while six AtIAAs and two AtGH3s were upregulated at the transcript level; moreover, the expression of three key class I KNOTTED-like homeobox (KNOX) genes was upregulated. In addition, by yeast two-hybrid screening of a chrysanthemum cDNA library, we found that CmTPL1-1 could interact with CmWOX4, CmLBD38 and CmLBD36, and these interactions were confirmed by bimolecular fluorescence complementation (BiFC) assays. Overall, we speculated that heterologous expression of CmTPL1-1 regulates plant growth and development by interacting with auxin signaling in Arabidopsis.