Viral IL-10 promotes cell proliferation and cell cycle progression via JAK2/STAT3 signaling pathway in nasopharyngeal carcinoma cells
Viral IL-10 promotes cell proliferation and cell cycle progression via JAK2/STAT3 signaling pathway in nasopharyngeal carcinoma cells
复制标题
病毒IL-10通过JAK2/STAT3信号通路促进鼻咽癌细胞增殖和细胞周期进程
DOI：
10.1002/bab.1856
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发表时间：
2019
影响因子：
2.8
通讯作者：
Xiaojiang Li
中科院分区：
文献类型：
--
作者：
Yanxin Ren;Jie Yang;Mei Li;Ning Huang;Yun Chen;Xiaojiang Li
EB病毒（EBV）与亚洲鼻咽癌（NPC）的发病率呈正相关。EBV感染后可产生多种蛋白质，包括病毒白细胞介素-10（vIL-10）。但vIL-10促进鼻咽癌细胞增殖和细胞周期的机制尚不清楚。progression.is本研究利用EB病毒阴性和阳性细胞株以及JAK 2/STAT 3信号通路抑制剂AG 490来研究vIL-10在鼻咽癌中的作用。CCK-8和流式细胞仪检测细胞增殖和细胞周期。Western blotting检测相关蛋白的表达水平。EB病毒阳性患者血清中vIL-10和IL-6浓度较高。IL-6的表达水平与vIL-10的浓度呈正相关。vIL-10可通过激活JAK 2/STAT 3信号通路上调IL-6蛋白水平，促进肿瘤细胞增殖和G1-S期转换。此外，EB病毒可以诱导细胞毒性T细胞的形成，而vIL-10可以阻断细胞毒性T细胞的功能。这些结果表明，vIL-10通过JAK 2/STAT 3信号通路促进鼻咽癌细胞增殖和细胞周期进程。
Epstein–Barr virus (EBV) is positively related to the morbidity of nasopharyngeal carcinoma (NPC) in Asia. After infection, EBV can produce several proteins, including viral nterleukin-10 (vIL-10). But the mechanism by which vIL-10 contributes to NPC cell proliferation and cell cycle progression.is not well understood. In this study, EBV negative and positive cell lines, and the JAK2/STAT3 signal pathway inhibitor AG490 were used to illustrate the role of vIL-10 in NPC. Cell proliferation and cell cycle were measured by CCK-8 and flow cytometry. The expression levels of related protein were measured by Western blotting. High concentrations of vIL-10 and IL-6 were found in the EBV positive patients. The expression level of IL-6 was positively related to the presence of concentration of vIL-10. vIL-10 can promote cancer cell proliferation and G1 to S phase transmission via upregulating.the IL-6 protein level by activating the JAK2/STAT3 signal pathway. Furthermore, EBV can induce the formation of cytotoxic T cells, whereas vIL-10 can block the function of.cytotoxic T cells. Taken together, these results suggest that vIL-10 promotes cell proliferation and cell cycle progression via JAK2/STAT3 signaling pathway in NPC.