A novel tissue-slice culture model for non-malignant human prostate
A novel tissue-slice culture model for non-malignant human prostate
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DOI：
10.1007/s00441-008-0602-z
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发表时间：
2008-06-01
影响因子：
3.6
通讯作者：
Ylikomi, T.
中科院分区：
文献类型：
--
作者：
Blauer, M.;Tammela, T. L.;Ylikomi, T.
建立了一种新的非肿瘤性人前列腺体外组织培养体系。精密切割的前列腺切片在培养板中培养，培养板上有一种新型的无血清混合物，基座是透气的。对培养标本进行细胞角蛋白18和14、雄激素受体(AR)、前列腺特异性抗原(PSA)、前列腺酸性磷酸酶(PAP)和内皮细胞标志物von Willebrand因子的免疫组织化学分析。在双氢睾酮(DHT)存在和不存在的情况下，也评估了上皮的存活率。在DHT存在的情况下，观察到令人满意的腺体细胞结构维持，大约一半的腺体表现为柱状或立方表型，并有完整的基底细胞层。在没有DHT的情况下，相应的百分比明显较低。在没有DHT的情况下，消退性改变和上皮细胞死亡的发生率显著增加。腺体细胞和间质细胞维持其表达AR的能力。PSA和PAP在整个培养过程中都有表达，尽管表达水平低于未培养的组织。内皮细胞的活性在不同样本之间有显著差异。在培养过程中，组织切片上覆盖着来自腺体的上皮细胞，腺体在组织切片过程中被切开。由具有管腔表型的细胞覆盖。目前的培养系统提供了一种新的体外环境，用于研究正常的人类前列腺生物学和病理生物学，并可能有助于避免与使用已建立的癌细胞系和动物模型有关的问题。
A novel tissue culture system was established for modeling the non-neoplastic human prostate in vitro. Precision-cut prostate slices were cultivated in culture plates with a gas-permeable base in a novel serun-free mixture. Cultivated specimens was evaluated by an immunohistochemical analysis of cytokeratins 18 and 14, androgen receptor (AR), prostate specific antigen (PSA), prostate acid phosphatase (PAP), and the endothelial cell marker von Willebrand factor. Epithelial viability in the presence and absence of dihydrotestosterone (DHT) was also assessed. Satisfactory maintenance of glandular cytoarchitecture was observed in the presence of DHT with approximately half of the glands displaying a columnar or cuboidal phenotype and an intact layer of basal cells. In the absence of DHT, the corresponding percentage was significantly lower. The occurrence of involutive changes and epithelial cell death was significantly higher in the absence of DHT. Glandular and stromal cells maintained their capacity to express AR. PSA and PAP were expressed throughout the culture period, albeit at a lower level than in uncultured tissue. The viability of endothelial cells differed markedly between individual samples. During culture, the tissue slices became covered with epithelial cells originating from glands that were cut open during tissue slicing. overlaid by cells with a luminal phenotype. The present culture system provides a novel in vitro setting in which to study normal human prostate biology and pathobiology and may help to obviate problems related to the use of established cancer cell lines and animal models.