Identification of the polypeptides encoded in the ATPase 6 gene and in the unassigned reading frames 1 and 3 of human mtDNA.
Identification of the polypeptides encoded in the ATPase 6 gene and in the unassigned reading frames 1 and 3 of human mtDNA.
复制标题
鉴定 ATP 酶 6 基因以及人类 mtDNA 未指定阅读框 1 和 3 中编码的多肽。
DOI：
10.1073/pnas.80.18.5535
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发表时间：
1983
影响因子：
11.1
通讯作者：
Doolittle,RF
中科院分区：
文献类型：
--
作者：
Chomyn,A;Mariottini,P;Gonzalez-Cadavid,N;Attardi,G;Strong,DD;Trovato,D;Riley,M;Doolittle,RF
针对从DNA序列预测的化学合成肽制备的抗体已用于鉴定ATP酶6基因和人mtDNA的未鉴定阅读框（URF）1和3中编码的多肽。特别是，针对ATP酶6阅读框编码的推定多肽的COOH末端九肽的抗体特异性免疫沉淀HeLa细胞线粒体翻译产物的组分17，该反应被特异性肽抑制。类似地，针对推定的URF 1产物的COOH-末端十一肽或针对推定的URF 3产物的COOH-末端七肽的抗体在特异性免疫沉淀线粒体翻译产物的组分12或组分24中是有效的。蛋白质17、12和24的大小，如从它们的电泳迁移率估计的，与它们分别是ATP酶6基因、URF 1和URF 3的产物相容。
Antibodies prepared against chemically synthesized peptides predicted from the DNA sequence have been used to identify the polypeptides encoded in the ATPase 6 gene and in unidentified reading frames (URFs) 1 and 3 of human mtDNA. In particular, antibodies directed against the COOH-terminal nonapeptide of the putative polypeptide encoded in the ATPase 6 reading frame immunoprecipitated specifically component 17 of the HeLa cell mitochondrial translation products, the reaction being inhibited by the specific peptide. Similarly, antibodies directed against the COOH-terminal undecapeptide of the putative URF1 product or against the COOH-terminal heptapeptide of the presumptive URF3 product were effective in immunoprecipitating specifically component 12 or, respectively, component 24 of the mitochondrial translation products. The sizes of proteins 17, 12, and 24, as estimated from their electrophoretic mobilities, are compatible with their being the products of the ATPase 6 gene, URF1, and URF3, respectively.