Characterization of Listeria monocytogenes isolated from retail foods.
Characterization of Listeria monocytogenes isolated from retail foods.
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DOI：
10.1016/j.ijfoodmicro.2006.07.010
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发表时间：
2007
影响因子：
5.4
通讯作者：
Yifan Zhang;Emily Yeh;G. Hall;J. Cripe;A. Bhagwat;J. Meng
中科院分区：
文献类型：
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作者：
Yifan Zhang;Emily Yeh;G. Hall;J. Cripe;A. Bhagwat;J. Meng
从零售即食（RTE）肉，生鸡肉和新鲜农产品中回收的单核细胞增生李斯特菌分离株，其特征在于使用PCR进行血清型鉴定，使用脉冲场凝胶电泳（PFGE）进行基因分型和抗菌药物敏感性测试。五湖鉴定单核细胞增多症血清群。其中1/2b、3b群68株（41%），4 b、4d、4 e群53株（32%），1/2a、3a群43株（26%），1/2c、3c群2株（1.2%），4a、4c群1株（0.6%）。PFGE共产生120种模式，与PCR血清型相关性良好。莫斯特湖单增菌对磺胺类药物耐药率为73%，四环素耐药率为8.4%，环丙沙星耐药率为1.8%。四环素耐药菌株具有接合转移性，在14株四环素耐药菌株及其接合子中均检测到泰特（M）基因。这些结果表明，L.食物中存在的单核细胞增多症多种多样，这些分离株对一种或多种抗生素的耐药性很常见。此外，由于血清型4 b是最常与人类大肠杆菌病有关的血清型，因此，所有食物类别中可能出现的血清型4 b均会引起公众卫生关注。
Listeria monocytogenes isolates recovered from retail ready-to-eat (RTE) meats, raw chickens and fresh produce were characterized by serogroup identification using PCR, genotyping using pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing. Five L. monocytogenes serogroups were identified. Of the 167 isolates 68 (41%) belonged to serogroup 1/2b, 3b; 53 (32%) belonged to serogroup 4b, 4d, 4e; 43 (26%) belonged to serogroup 1/2a, 3a; 2 (1.2%) belonged to serogroup 1/2c, 3c; and 1 (0.6%) belonged to serogroup 4a, 4c. PFGE generated 120 patterns which correlated well with PCR serogrouping. Most L. monocytogenes isolates were resistant to sulfonamide (73%) and some were resistant to tetracycline (8.4%) and ciprofloxacin (1.8%). Tetracycline resistance was conjugatively transferable and the tet(M) gene was identified in 14 tetracycline-resistant isolates as well as their transconjugants. These findings indicate that L. monocytogenes present in food were diverse, and that resistance to one or more antibiotics among these isolates was common. In addition, the presence of potential serotype 4b in all food categories is of public health concern, as serotype 4b has been the serotype most frequently associated with human listeriosis.