PCR Technology : Current Innovations
PCR Technology : Current Innovations
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PCR 技术：当前的创新
DOI：
10.1201/b12376
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发表时间：
1994
影响因子：
--
通讯作者：
A. Griffin
中科院分区：
文献类型：
--
作者：
H. Griffin;A. Griffin
PCR作为分子生物学中的日常使用技术（J.- P. Charlieu）。PCR引物的设计（A。Sharrocks）。PCR产物的纯化（L. Mezei和D. Storts）。克隆PCR产物（L. Mezei和D. Storts）。通过单特异性引物PCR扩增未知侧翼DNA（J. Stappert）。通过PCR产生标记的DNA探针（G. Konat）。通过PCR（E.P.H.雅普等人）。λ和粘粒文库的直接PCR筛选（H.G. Griffin）。体外产生多位点定向突变的方法（J. Stappert）。使用PCR的诱变（B. Tao）。ds和ss PCR产物的直接自动DNA测序（A. Lew）。通过聚合酶链反应区分几乎相同的DNA序列（J.- P. Charlieu）。通过PCR（E.P.H.雅普等人）。PCR产物中的限制性位点多态性（MRSPs）用于遗传差异生物的快速鉴定和分类（D。拉尔夫和M. McClelland）。通过PCR检测Alu重复序列3'末端的多态性DNA序列（J.- P. Charlieu）。连接锚定PCR（A. Troutt）。PCR-限制性稀释分析（A. Troutt）。使用甲酰胺和低温从全血直接PCR（A. Lew）。免疫PCR：一种通用的PCR靶纯化方法（A。Lew）。PCR产物的非同位素单链构象多态性（SSCP）分析（E.P.H.）雅普等人）。PCR-RAPD分析在植物分类与进化中的应用。Demeke和R. P.亚当斯）。植物随机扩增多态性DNA（RAPD）分析中DNA提取和PCR方法的优化（K。Yu和K.P. Pauls）。利用RAPD分析标记基因和确定杂种优势植物群体的亲缘关系，以四倍体拟南芥为例（K。Yu和K.P. Pauls）。PCR过程中的DNA扩增（A. Vartapetian）。用于体外DNA扩增的热稳定DNA聚合酶（A. Bej和M. Mahbubani）。序列标记位点（STS）内容基因组作图中的PCR（A。Srivastava）。PCR中的假阳性和污染（E.P.H.）雅普等人）。聚合酶链反应（PCR）技术在法医学分析中的应用。Presley）。PCR扩增DNA标记在个体鉴定中的应用。Peltonen和A. Sajantila）。法医学中的PCR（V. Pascali）。聚合酶链反应在临床诊断中的应用（A。Bej和M. Mahbubani）。聚合酶链反应（PCR）体外DNA扩增技术在环境微生物学中的应用（A。Bej和M. Mahbubani）。应用聚合酶链反应检测食源性致病微生物。Jones和A. Bej）。
PCR as a Daily Used Technique in Molecular Biology (J.-P. Charlieu). The Design of Primers for PCR (A. Sharrocks). Purification of PCR Products (L. Mezei and D. Storts). Cloning PCR Products (L. Mezei and D. Storts). Amplifying of Unknown Flanking DNA by Single-Specific-Primer PCR (J. Stappert). Generation of Labeled DNA Probes by PCR (G. Konat). Nonisotopic Probe Generation by PCR (E.P.H. Yap, et al.). Direct PCR Screening of Lambda and Cosmid Libraries (H.G. Griffin). Methods for Generating Multiple Site Directed Mutations In Vitro (J. Stappert). Mutagenesis Using PCR (B. Tao). Direct Automated DNA Sequencing of ds and ss PCR Products (A. Lew). Distinction between Almost Identical DNA Sequences by Polymerase Chain Reaction (J.-P. Charlieu). Detection of Mutations by PCR (E.P.H. Yap, et al.). Mapped Restriction Site Polymorphisms (MRSPs) in PCR Products for Rapid Identification and Classification of Genetically Distinct Organisms (D. Ralph and M. McClelland). Detection of Polym orphic DNA Sequences at the 3' end of Alu Repeats by PCR (J.-P. Charlieu). Ligation-Anchored PCR (A. Troutt). PCR-Limiting Dilution Analysis (A. Troutt). Direct PCR from Whole Blood Using Formamide and Low Temperatures (A. Lew). Immuno-PCR: A Generic Method for Purifying Target for PCR (A. Lew). Nonisotopic Single Stranded Conformation Polymorphism (SSCP) Analysis of PCR Products (E.P.H. Yap, et al.). The Use of PCR-RAPD Analysis in Plant Taxonomy and Evolution (T. Demeke and R.P. Adams). Optimization of DNA Extraction and PCR Procedures for Random Amplified Polymorphic DNA (RAPD) Analysis in Plants (K. Yu and K.P. Pauls). The Use of RAPD Analysis to Tag Genes and Determine Relatedness in Heterologous Plant Populations Using Tetraploid Alfalfa as an Example (K. Yu and K.P. Pauls). DNA Recombination in the Course of PCR (A. Vartapetian). Thermostable DNA Polymerases for In Vitro DNA Amplifications (A. Bej and M. Mahbubani). PCR in Sequence-Tagged Site (STS) Content Genome Mapping (A. Srivastava). False Positives and Contamination in PCR (E.P.H. Yap, et al.). The Application of Polymerase Chain Reaction (PCR) Based Technologies to Forensic Analysis (L. Presley). Application of PCR Amplified DNA Markers in Identification of Individuals (L. Peltonen and A. Sajantila). PCR in Forensic Science (V. Pascali). Applications of Polymerase Chain Reaction Methodology in Clinical Diagnostics (A. Bej and M. Mahbubani). Applications of the Polymerase Chain Reaction (PCR) In Vitro DNA Amplification Method in Environmental Microbiology (A. Bej and M. Mahbubani). Detection of Foodborne Microbial Pathogens Using Polymerase Chain Reaction (D. Jones and A. Bej).