The cotton fiber transcriptome
The cotton fiber transcriptome
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DOI：
10.1111/j.1399-3054.2005.00514.x
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发表时间：
2005-07
影响因子：
6.4
通讯作者：
T. Wilkins;A. Arpat
中科院分区：
文献类型：
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作者：
T. Wilkins;A. Arpat
在植物中已知的最长细胞类型中，棉纤维是经济上重要的种子毛状体，为研究基本生物过程提供了独特的单细胞模型系统。功能基因组方法已用于表征棉花纤维转录组响应发育信号的动态变化，这些发育信号在单细胞水平上控制纤维形态发生。纤维转录组的遗传复杂性非常高，占棉花基因组基因的45-50%之多。除了大量不同的组成型表达基因外，转录组的表达谱还揭示了两种发育调节的阶段特异性表达模式，这些模式定义了相对于次生细胞壁生物发生而言的初生细胞壁（PCW）合成过程中细胞的快速伸长。在发育棉纤维的过程中，许多参与 PCW 合成和膨压驱动的细胞扩张的纤维基因以与生长速率平行的方式差异表达。棉花纤维转录组的表征可直接应用于农业生物技术和分子育种计划，以提高产量和纤维质量。
Among the longest cell types known in plants, cotton fibers are economically important seed trichomes that provide a unique single-celled model system for studying fundamental biological processes. Functional genomic approaches have served to characterize dynamic changes to the cotton fiber transcriptome in response to developmental signals that control fiber morphogenesis at the level of a single cell. The genetic complexity of the fiber transcriptome is very high and accounts for as much as 45-50% of the genes in the cotton genome. In addition to a large diverse group of constitutively expressed genes, expression profiling of the transcriptome revealed two developmentally regulated stage-specific expression patterns that define rapid cell elongation during primary cell wall (PCW) synthesis relative to secondary cell wall biogenesis. In developing cotton fibers, many fiber genes involved in PCW synthesis and turgor-driven cell expansion are differentially expressed in a manner that parallels the growth rate. Characterization of the cotton fiber transcriptome has immediate applications in agricultural biotechnology and molecular breeding programs geared toward the genetic improvement of yield and fiber quality.