3D plasmonic nanobowl platform for the study of exosomes in solution
3D plasmonic nanobowl platform for the study of exosomes in solution
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DOI：
10.1039/c5nr01333j
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发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Wachsmann-Hogiu, Sebastian
中科院分区：
文献类型：
--
作者：
Lee, Changwon;Carney, Randy P.;Wachsmann-Hogiu, Sebastian
薄膜镀银纳米碗表面增强拉曼光谱（SERS）底物用于捕获溶液中的外泌体，用于SERS测量，可以提供完整和破裂外泌体的生化分析。通过总外泌体分离试剂（TEIR）和超离心（UC）对SKOV3细胞系的外泌体进行了分析。通过TEIR衍生的外泌体光谱由TEIR试剂盒的信号特征主导，需要减去所有测量。用主成分分析（PCA）对外泌体溶液干燥过程中不同时间记录的SERS光谱差异进行统计分析。在干燥过程开始时，外泌体的SERS光谱表现出脂质和蛋白质的特征峰。在随后的干燥过程中，新的SERS峰出现，表明最初完整的外泌体随着时间的推移而破裂。这种随时间变化的SERS峰能够分析外泌体膜内容物和外泌体内部的内容物。
Thin silver film coated nanobowl Surface Enhanced Raman Spectroscopy (SERS) substrates are used to capture exosomes in solution for SERS measurements that can provide biochemical analysis of intact and ruptured exosomes. Exosomes derived via Total Exosome Isolation Reagent (TEIR) as well as ultracentrifugation (UC) from the SKOV3 cell line were analyzed. Spectra of exosomes derived via TEIR are dominated by a signal characteristic for the TEIR kit that needs to be subtracted for all measurements. Differences in SERS spectra recorded at different times during the drying of the exosome solution are statistically analyzed with Principal Component Analysis (PCA). At the beginning of the drying process, SERS spectra of exosomes exhibit peaks characteristic for both lipids and proteins. Later on during the drying process, new SERS peaks develop, suggesting that the initially intact exosome ruptures over time. This time-dependent evolution of SERS peaks enables analysis of exosomal membrane contents and the contents inside the exosomes.