Characterization of adherens junction protein expression and localization in pituitary cell networks
Characterization of adherens junction protein expression and localization in pituitary cell networks
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DOI：
10.1677/joe-09-0153
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发表时间：
2009-09-01
影响因子：
4
通讯作者：
Coutry, Nathalie
中科院分区：
文献类型：
--
作者：
Chauvet, Norbert;El-Yandouzi, Taoufik;Coutry, Nathalie
随着我们认识到滤泡星状细胞（FS）和生长激素细胞群形成大规模的三维同型网络，我们对垂体前叶组织的看法已经改变。这种形态细胞组织可以优化垂体内的通讯，促进适应生理需要的协调脉动分泌。本研究的目的是鉴定参与这些细胞-细胞网络内形成和潜在功能组织和/或信号传导的分子。在这里，我们重点关注一类细胞粘附分子，即钙粘蛋白，因为在 GH 细胞网络中检测到了 β-连环蛋白。我们通过 qPCR 和免疫组织化学表征了它们的细胞表达和分布。我们还检查了它们的表达是否可以在垂体组织重塑过程中受到调节。小鼠垂体前叶具有受限的、细胞类型特异性的钙粘蛋白表达：钙粘蛋白-11仅在TSH细胞中表达； N-钙粘蛋白表现出普遍存在的表达模式，但在内分泌细胞类型之间表达水平不同； E-钙粘蛋白仅限于 FS 细胞之间的同型接触；而cadherin-18在生长激素细胞和FS细胞中均表达。因此，每种细胞类型都呈现不同钙粘蛋白亚群的明确组合表达。这种细胞类型特异性钙粘蛋白表达谱在发育早期出现，并在出生后发育过程中经历重大变化。这些结果表明，垂体内部存在基于钙粘蛋白表达确定模式的细胞间接触信号传导，这可能在垂体细胞同型网络的形成和命运过程中的细胞识别中发挥至关重要的作用。内分泌学杂志 (2009) 202, 375-387
Our view of anterior pituitary organization has been altered with the recognition that folliculo-stellate (FS) and somatotroph cell populations form large-scale three-dimensional homotypic networks. This morphological cellular organization may optimize communication within the pituitary gland promoting coordinated pulsatile secretion adapted to physiological needs. The aim of this study was to identify the molecules involved in the formation and potential functional organization and/or signaling within these cell-cell networks. Here, we have focused on one class of cell adhesion molecules, the cadherins, since beta-catenin has been detected in the GH cell network. We have characterized, by qPCR and immunohistochemistry, their cellular expression and distribution. We have also examined whether their expression could be modulated during pituitary tissue remodeling. The mouse anterior pituitary has a restricted and cell-type specific repertoire of cadherin expression: cadherin-11 is exclusively expressed in TSH cells; N-cadherin displays a ubiquitous expression pattern but with different levels of expression between endocrine cell types; E-cadherin is restricted to homotypic contacts between FS cells; while cadherin-18 is expressed both in somatotrophs and FS cells. Thus, each cell type presents a defined combinatorial expression of different subsets of cadherins. This cell-type specific cadherin expression profile emerges early during development and undergoes major changes during postnatal development. These results suggest the existence within the interior pituitary of cell-cell contact signaling based on a defined pattern of cadherin expression, which may play a crucial role in cellular recognition during the formation and fate of pituitary cell homotypic networks. journal of Endocrinology (2009) 202, 375-387